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Quantitative Temporal Viromics.

Published version
Peer-reviewed

Type

Article

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Authors

Fletcher-Etherington, Alice  ORCID logo  https://orcid.org/0000-0001-7313-9247
Weekes, Michael P 

Abstract

The abundance, localization, modifications, and protein-protein interactions of many host cell and virus proteins can change dynamically throughout the course of any viral infection. Studying these changes is critical for a comprehensive understanding of how viruses replicate and cause disease, as well as for the development of antiviral therapeutics and vaccines. Previously, we developed a mass spectrometry-based technique called quantitative temporal viromics (QTV), which employs isobaric tandem mass tags (TMTs) to allow precise comparative quantification of host and virus proteomes through a whole time course of infection. In this review, we discuss the utility and applications of QTV, exemplified by numerous studies that have since used proteomics with a variety of quantitative techniques to study virus infection through time.

Description

Keywords

host microbial interactions, innate immunity, proteomics, viral proteins, viruses, Mass Spectrometry, Proteome, Proteomics, Viral Proteins, Viruses

Journal Title

Annu Rev Virol

Conference Name

Journal ISSN

2327-056X
2327-0578

Volume Title

8

Publisher

Annual Reviews
Sponsorship
Wellcome Trust (108070/Z/15/Z)