Multivariate data analysis and metabolic profiling of artemisinin and related compounds in high yielding varieties of Artemisia annua field-grown in Madagascar.
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Abstract
An improved liquid chromatography-tandem mass spectrometry (LC-MS/MS) protocol for rapid analysis of co-metabolites of A. annua in raw extracts was developed and extensively characterized. The new method was used to analyse metabolic profiles of 13 varieties of A. annua from an in-field growth programme in Madagascar. Several multivariate data analysis techniques consistently show the association of artemisinin with dihydroartemisinic acid. These data support the hypothesis of dihydroartemisinic acid being the late stage precursor to artemisinin in its biosynthetic pathway.
Description
Keywords
Artemisia annua, Metabolic profiling, Multivariate data analysis, Artemisia annua, Madagascar, Metabolomics, Multivariate Analysis, Plant Extracts, Plant Leaves, Statistics as Topic
Journal Title
J Pharm Biomed Anal
Conference Name
Journal ISSN
0731-7085
1873-264X
1873-264X
Volume Title
117
Publisher
Elsevier BV
Publisher DOI
Sponsorship
The research leading to these results has received funding from Engineering and Physical Sciences Research Council project “Closed Loop Optimization for Sustainable Chemical Manufacture” [EP/L003309/1].