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Functionally Distinct Subsets of Lineage-Biased Multipotent Progenitors Control Blood Production in Normal and Regenerative Conditions.



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Pietras, Eric M 
Reynaud, Damien 
Kang, Yoon-A 
Carlin, Daniel 
Calero-Nieto, Fernando J 


Despite great advances in understanding the mechanisms underlying blood production, lineage specification at the level of multipotent progenitors (MPPs) remains poorly understood. Here, we show that MPP2 and MPP3 are distinct myeloid-biased MPP subsets that work together with lymphoid-primed MPP4 cells to control blood production. We find that all MPPs are produced in parallel by hematopoietic stem cells (HSCs), but with different kinetics and at variable levels depending on hematopoietic demands. We also show that the normally rare myeloid-biased MPPs are transiently overproduced by HSCs in regenerating conditions, hence supporting myeloid amplification to rebuild the hematopoietic system. This shift is accompanied by a reduction in self-renewal activity in regenerating HSCs and reprogramming of MPP4 fate toward the myeloid lineage. Our results support a dynamic model of blood development in which HSCs convey lineage specification through independent production of distinct lineage-biased MPP subsets that, in turn, support lineage expansion and differentiation.



Animals, Cell Differentiation, Cell Lineage, Cellular Reprogramming, Gene Expression, Hematopoiesis, Hematopoietic Stem Cells, Lymphoid Progenitor Cells, Mice, Mice, Inbred C57BL, Mice, Transgenic, Models, Biological, Multipotent Stem Cells, Myeloid Progenitor Cells, Regeneration

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Cell Stem Cell

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Cell Press
Cancer Research Uk (None)
Leukaemia & Lymphoma Research (12029)
Wellcome Trust (097922/Z/11/Z)
Medical Research Council (MC_PC_12009)
Leukemia & Lymphoma Society (7001-12)
Biotechnology and Biological Sciences Research Council (BB/I00050X/1)
Medical Research Council (MR/M008975/1)
Wellcome Trust (097922/Z/11/B)
This work was supported by NIH awards F32HL106989 and K01DK098315 to E.M.P, grants from Leukaemia and Lymphoma Research, Cancer Research UK and core support by the Wellcome Trust to B.G.; and NIH grant R01HL092471, Rita Allen Scholar Award and Leukemia Lymphoma Society Scholar Award to E.P.