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Recognizing the reagent microbiome

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cam.issuedOnline2018-07-25
dc.contributor.authorde Goffau, Marcus
dc.contributor.authorLager, Susanne
dc.contributor.authorSalter, Susannah
dc.contributor.authorWagner, Josef
dc.contributor.authorKronbichler, Andreas
dc.contributor.authorJones, David Stephen
dc.contributor.authorPeacock, Sharon J
dc.contributor.authorSmith, GCS
dc.contributor.orcidde Goffau, Marcus [0000-0001-7032-1852]
dc.contributor.orcidSalter, Susannah [0000-0003-3898-8504]
dc.contributor.orcidKronbichler, Andreas [0000-0002-2945-2946]
dc.contributor.orcidCharnock-Jones, Stephen [0000-0002-2936-4890]
dc.contributor.orcidPeacock, Sharon [0000-0002-1718-2782]
dc.contributor.orcidSmith, Gordon [0000-0003-2124-0997]
dc.date.accessioned2018-10-10T05:20:11Z
dc.date.available2018-10-10T05:20:11Z
dc.date.issued2018-08
dc.description.abstractA noticeable part of the microbiome literature, especially that working with low-biomass samples, is plagued by reagent contamination. Here we describe visual, statistical, methodical and ecological techniques to facilitate recognition of signals which represent contamination.
dc.description.sponsorshipThe work was supported by the Medical Research Council (United Kingdom; G1100221) and the National Institute for Health Research (NIHR) Cambridge Comprehensive Biomedical Research Centre (Women’s Health theme).
dc.identifier.doi10.17863/CAM.30841
dc.identifier.eissn2058-5276
dc.identifier.issn1740-1526
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/283475
dc.language.isoeng
dc.publisherSpringer Nature
dc.publisher.urlhttp://dx.doi.org/10.1038/s41564-018-0202-y
dc.subjectDNA Contamination
dc.subjectDatabases, Genetic
dc.subjectGastrointestinal Microbiome
dc.subjectHigh-Throughput Nucleotide Sequencing
dc.subjectHumans
dc.subjectIndicators and Reagents
dc.subjectMicrobiota
dc.subjectRNA, Ribosomal, 16S
dc.titleRecognizing the reagent microbiome
dc.typeArticle
dcterms.dateAccepted2018-04-27
prism.endingPage853
prism.publicationNameNature Reviews Microbiology
prism.startingPage851
prism.volume3
pubs.funder-project-idMedical Research Council (MR/K021133/1)
pubs.funder-project-idCambridge University Hospitals NHS Foundation Trust (CUH) (146281)
pubs.funder-project-idMedical Research Council (G1100221)
pubs.funder-project-idMedical Research Council (G1100221/1)
rioxxterms.licenseref.startdate2018-04-27
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.typeJournal Article/Review
rioxxterms.versionAM
rioxxterms.versionofrecord10.1038/s41564-018-0202-y

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