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Enhanced surface nano-analytics of transient biomolecular processes

Published version
Peer-reviewed

Type

Article

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Authors

Miller, Alyssa Marie 
Toprakcioglo, Zenon 

Abstract

Fundamental knowledge of the physical and chemical properties of biomolecules is key to understanding molecular processes in health and disease. Bulk and single-molecule analytical methods provide rich information on biomolecules, but often require high concentrations and sample preparation away from physiologically relevant conditions. Here, we present the development and application of a lab-on-a-chip spray approach which combines rapid sample preparation, mixing and deposition to integrate with a range of nano-analytical methods in chemistry and biology, providing enhanced spectroscopic sensitivity and single molecule spacial resolution. We demonstrate that this method enables multidimensional study of heterogenous biomolecular systems over multiple length scales by nanoscopy and vibrational spectroscopy. We then illustrate the capabilities of this platform by capturing and analysing the structural conformations of transient oligomeric species formed at the early stages of the self-assembly of -synuclein, which are associated with the onset of Parkinson’s disease.

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Keywords

Journal Title

Science Advances

Conference Name

Journal ISSN

2375-2548
2375-2548

Volume Title

Publisher

American Association for the Advancement of Science
Sponsorship
ERC, EPSRC and Newman Foundation