Research data supporting "Nanopore detection of single-nucleotide RNA mutations and modifications with programmable nanolatches".

Name: Research data supporting "Nanopore detection of single-nucleotide RNA mutations and modifications with programmable nanolatches".
Published: 2025-06-11T08:15:54Z
Keywords: nanopore sensing, RNA modification, RNA mutation

Li, Yunxuan; Meng, Siong Chen; Wang, Yesheng; Platnich, Casey; Earle, Max; Mylona, Elli; Naydenova, Plamena; Baker, Stephen; Zhu, Jinbo; Keyser, Ulrich

doi:https://doi.org/10.17863/CAM.118402

Research data supporting "Nanopore detection of single-nucleotide RNA mutations and modifications with programmable nanolatches".

Repository URI

https://www.repository.cam.ac.uk/handle/1810/384372

Repository DOI

https://doi.org/10.17863/CAM.118402

Files

Source Data.zip (1.32 GB)

Type

Dataset

Authors

Li, Yunxuan

https://orcid.org/0009-0005-8362-5173

Description

The dataset includes: (a) nanopore ionic current traces recorded using LabVIEW from an Axopatch 200B amplifier; (b) Sanger sequencing data provided by the Cambridge Biochemistry Sequencing Team; and (c) gel images; for all figures presented in the paper, including main, extended data, and supplementary figures.

Software / Usage instructions

LabVIEW code was used to collect and process data recorded with an Axopatch 200B amplifier. Individual translocation events were extracted from the raw ionic current traces and saved as .tdms binary files, which are compatible with analysis in Python. Sanger sequencing data are provided as .ab1 files, which can be viewed and analyzed using SnapGene.