Isolating Chromosomes of the Komodo Dragon: New Tools for Comparative Mapping and Sequence Assembly.


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Type
Article
Change log
Authors
Iannucci, Alessio 
Altmanová, Marie 
Ciofi, Claudio 
Ferguson-Smith, Malcolm  ORCID logo  https://orcid.org/0000-0001-9372-1381
Pereira, Jorge C 
Abstract

We developed new tools to build a high-quality chromosomal map of the Komodo dragon (Varanus komodoensis) available for cross-species phylogenomic analyses. First, we isolated chromosomes by flow sorting and determined the chromosome content of each flow karyotype peak by FISH. We then isolated additional Komodo dragon chromosomes by microdissection and amplified chromosome-specific DNA pools. The chromosome-specific DNA pools can be sequenced, assembled, and mapped by next-generation sequencing technology. The chromosome-specific paint probes can be used to investigate karyotype evolution through cross-species chromosome painting. Overall, the set of chromosome-specific DNA pools of V. komodoensis provides new tools for detailed phylogenomic analyses of Varanidae and squamates in general.

Description
Keywords
Chromosome flow sorting, Laser capture, Microdissection, Molecular cytogenetics, Varanus komodoensis, Animals, Chromosome Banding, Chromosome Mapping, Chromosome Painting, Chromosomes, DNA, DNA Probes, Female, High-Throughput Nucleotide Sequencing, Karyotype, Lizards
Journal Title
Cytogenet Genome Res
Conference Name
Journal ISSN
1424-8581
1424-859X
Volume Title
157
Publisher
S. Karger AG
Sponsorship
none