Research data in support of "Internal Phase Separation in Synthetic DNA Condensates"
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Change log
Description
Data to support paper 'Internal phase separation in synthetic DNA condensates'. Each folder for main figures (1-6) is accompanied by a corresponding README file.
- Confocal micrographs for DNA condensates with equal parts of A to B nanostars.
- Confocal micrographs for DNA condensates with all ratios of A to B tested.
- Images of condensates selected for analysis (highlighted) and resulting measurements of partition coefficients, contact angles, and interfacial tension ratios.
- Epifluorescence micrographs and measurements of mean fluorescence intensity, standard deviation of fluorescence intensity, and melting temperatures for experiments of melting and annealing DNA condensates.
- Confocal micrographs for DNA condensates annealed with different protocols.
- Images of condensates annealed under different conditions selected for analysis (highlighted) and resulting measurements of partition coefficients, contact angles, and interfacial tension ratios.
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Software / Usage instructions
Image analysis and processing was carried out in MATLAB. Plots were generated using MATLAB and PYTHON. Microscopy images (.tif and .lif) can be opened using Fiji.
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Except where otherwised noted, this item's license is described as https://creativecommons.org/licenses/by/4.0/
Sponsorship
BBSRC (BB/X010228/1)
Royal Society (URF\R\221009)
Royal Society (URF\R\221009)
L.D.M., D.A.T. and L.M. acknowledge support from the European Research Council (ERC) under the Horizon 2020 research and innovation programme (ERC-STG No 851667 - NANOCELL). L.D.M. acknowledges support from a Royal Society University Research Fellowship (UF160152,
URF\R\221009). R.R.S. acknowledges funding from the Biotechnology and Biological Sciences Research Council through a BBSRC Discovery Fellowship (BB/X010228/1). E.F. and D.O. acknowledge support from the U.S. National Science Foundation (NSF) grant FMRG: Bio award 2134772, by the Alfred Sloan Foundation through awards G-2021-16831 and G-2024-22575.