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Folic acid-tagged protein nanoemulsions loaded with CORM-2 enhance the survival of mice bearing subcutaneous A20 lymphoma tumors.

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Loureiro, Ana 
Bernardes, Gonçalo JL 
Shimanovich, Ulyana 
Sárria, Marisa P 
Nogueira, Eugénia 


UNLABELLED: Folic Acid (FA)-tagged protein nanoemulsions were found to be preferentially internalized on B-cell lymphoma cell line (A20 cell line), which, for the first time, is reported to express folate receptor (FR)-alpha. Carbon monoxide releasing molecule-2 (CORM-2) was incorporated in the oil phase of the initial formulation. FA-functionalized nanoemulsions loaded with CORM-2 exhibited a considerable antitumor effect and an increased survival of BALB/c mice bearing subcutaneous A20 lymphoma tumors. The developed nanoemulsions also demonstrated to be well tolerated by these immunocompetent mice. Thus, the results obtained in this study demonstrate that FA-tagged protein nanoemulsions can be successfully used in cancer therapy, with the important ability to delivery drugs intracellularly. FROM THE CLINICAL EDITOR: In this research, the authors developed folic acid tagged nanoemulsions containing a carbon monoxide releasing protein molecule for targeted cancer cell treatment. In-vitro and in-vivo experiments showed efficacy against B-cell lymphoma cells. The same nanocarrier platform could be applied to other tumor cells expressing folate receptors on the cell surface.



CORM-2, Folic acid, Protein nanoemulsions, Specific uptake, Targeted drug delivery, Animals, Antineoplastic Agents, Cell Line, Tumor, Drug Carriers, Drug Delivery Systems, Female, Folate Receptors, GPI-Anchored, Folic Acid, Humans, Lymphoma, Mice, Inbred BALB C, Organometallic Compounds, Serum Albumin, Bovine

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Elsevier BV