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Biostack: Nontoxic Metabolite Detection from Live Tissue

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dc.contributor.authorStrakosas, Xenofon
dc.contributor.authorDonahue, Mary J.
dc.contributor.authorHama, Adel
dc.contributor.authorBraendlein, Marcel
dc.contributor.authorHuerta, Miriam
dc.contributor.authorSimon, Daniel T.
dc.contributor.authorBerggren, Magnus
dc.contributor.authorMalliaras, George G.
dc.contributor.authorOwens, Roisin M.
dc.contributor.orcidStrakosas, Xenofon [0000-0001-5757-8565]
dc.date.accessioned2021-11-06T06:07:55Z
dc.date.available2021-11-06T06:07:55Z
dc.date.issued2021-11-05
dc.date.submitted2021-04-27
dc.date.updated2021-11-06T06:07:55Z
dc.descriptionFunder: ANR‐T
dc.descriptionFunder: IONOSENSE
dc.description.abstractAbstract: There is increasing demand for direct in situ metabolite monitoring from cell cultures and in vivo using implantable devices. Electrochemical biosensors are commonly preferred due to their low‐cost, high sensitivity, and low complexity. Metabolite detection, however, in cultured cells or sensitive tissue is rarely shown. Commonly, glucose sensing occurs indirectly by measuring the concentration of hydrogen peroxide, which is a by‐product of the conversion of glucose by glucose oxidase. However, continuous production of hydrogen peroxide in cell media with high glucose is toxic to adjacent cells or tissue. This challenge is overcome through a novel, stacked enzyme configuration. A primary enzyme is used to provide analyte sensitivity, along with a secondary enzyme which converts H2O2 back to O2. The secondary enzyme is functionalized as the outermost layer of the device. Thus, production of H2O2 remains local to the sensor and its concentration in the extracellular environment does not increase. This “biostack” is integrated with organic electrochemical transistors to demonstrate sensors that monitor glucose concentration in cell cultures in situ. The “biostack” renders the sensors nontoxic for cells and provides highly sensitive and stable detection of metabolites.
dc.identifier.doi10.17863/CAM.77844
dc.identifier.issn2198-3844
dc.identifier.otheradvs202101711
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/330401
dc.languageen
dc.subjectResearch Article
dc.subjectResearch Articles
dc.subjectbiofunctionalization
dc.subjectbiosensors
dc.subjectglucose monitoring
dc.subjectorganic bioelectronics
dc.subjectorganic electrochemical transistors
dc.titleBiostack: Nontoxic Metabolite Detection from Live Tissue
dc.typeArticle
prism.publicationNameAdvanced Science
pubs.funder-project-idEuropean Research Council (ERC‐2010‐StG, 258966)
pubs.funder-project-idEuropean Research Council (258966)
rioxxterms.licenseref.urihttp://creativecommons.org/licenses/by/4.0/
rioxxterms.versionVoR
rioxxterms.versionofrecord10.1002/advs.202101711

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