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Human Primordial Germ Cell-Like Cell Induction from Pluripotent Stem Cells by SOX17 and PRDM1 Expression

Accepted version
Peer-reviewed

Change log

Authors

Irie, Naoko 
Kobayashi, Toshihiro 
Azim Surani, M 

Abstract

Human primordial germ cell (PGC) development initiates about 2 weeks after fertilization during embryogenesis. Unique molecular events follow, including epigenetic resetting, to establish functional gametes (egg and sperm). Due to the inaccessibility of human embryos, it is essential to have an amenable experimental platform to investigate the mechanisms and potential dysfunctions of the events. We previously established a PGC-like cell (PGCLC) differentiation method using human pluripotent stem cells (PSCs) via induction of precursor cells followed by stimulation with a cytokine cocktail including BMP. We also revealed that the expression of PGC specifiers, SOX17 and PRDM1, can robustly induce PGCLCs from PSCs without the cytokines. The balance of SOX17 and PRDM1 is critical for germ cell fate since the two factors also regulate endoderm differentiation. Here we describe a detailed procedure for PGCLC differentiation with the balanced induction of SOX17 and PRDM1. The protocol can be used for PGC induction in other mammalian species exhibiting PGCs with SOX17 expression. Together, these studies will advance the understanding of germ cell biology and its applications in reproductive technology and medicine.

Description

Title

Human Primordial Germ Cell-Like Cell Induction from Pluripotent Stem Cells by SOX17 and PRDM1 Expression

Keywords

Human pluripotent stem cells, Human primordial germ cells, PRDM1, Primordial germ cell-like cells, SOX17, Animals, Humans, Male, Semen, Pluripotent Stem Cells, Cell Differentiation, Germ Cells, Embryo, Mammalian, Mammals, SOXF Transcription Factors, Positive Regulatory Domain I-Binding Factor 1

Is Part Of

Methods in Molecular Biology

Book type

Edited volume

Publisher

Springer US

ISBN

9781071636978
Sponsorship
Wellcome Trust (096738/Z/11/Z)
Wellcome Trust (209475/Z/17/Z)