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Targeted knockdown of ribulose-1, 5-bisphosphate carboxylase-oxygenase in rice mesophyll cells.

Published version
Peer-reviewed

Type

Article

Change log

Authors

Maheshwari, Chirag 
Coe, Robert A 
Karki, Shanta 
Covshoff, Sarah 
Tapia, Ronald 

Abstract

We generated antisense constructs targeting two of the five Rubisco small subunit genes (OsRBCS2 and 4) which account for between 30-40 % of the RBCS transcript abundance in leaf blades. The constructs were driven by a maize phosphoenolpyruvate carboxylase (PEPC) promoter known to have enriched expression in mesophyll cells (MCs). In the resulting lines leaf, Rubisco protein content was reduced by between 30-50 % and CO2 assimilation rate was limited under photorespiratory and non-photorespiratory conditions. A relationship between Rubisco protein content and CO2 assimilation rate was found. This was associated with a significant reduction in dry biomass accumulation and grain yield of between 37-70%. In addition to serving as a resource for reducing Rubisco accumulation in a cell-preferential manner, these lines allow us to characterize gene function and isoform specific suppression on photosynthesis and growth. Our results suggest that the knockdown of multiple genes is required to completely reduce Rubisco accumulation in MCs.

Description

Keywords

Antisense, C(4) rice, Oryza sativa, RBCS, Rubisco, Gene Knockdown Techniques, Mesophyll Cells, Oryza, Photosynthesis, Plant Leaves, Ribulose-Bisphosphate Carboxylase

Journal Title

J Plant Physiol

Conference Name

Journal ISSN

0176-1617
1618-1328

Volume Title

260

Publisher

Elsevier BV
Sponsorship
Bill & Melinda Gates Foundation (via International Rice Research Institute (IRRI)) (unknown)