Recycling of memory B cells between germinal center and lymph node subcapsular sinus supports affinity maturation to antigenic drift.


Type
Article
Change log
Authors
Zhang, Yang 
Garcia-Ibanez, Laura 
Pike, Jeremy A 
Abstract

Infection or vaccination leads to the development of germinal centers (GC) where B cells evolve high affinity antigen receptors, eventually producing antibody-forming plasma cells or memory B cells. Here we follow the migratory pathways of B cells emerging from germinal centers (BEM) and find that many BEM cells migrate into the lymph node subcapsular sinus (SCS) guided by sphingosine-1-phosphate (S1P). From the SCS, BEM cells may exit the lymph node to enter distant tissues, while some BEM cells interact with and take up antigen from SCS macrophages, followed by CCL21-guided return towards the GC. Disruption of local CCL21 gradients inhibits the recycling of BEM cells and results in less efficient adaption to antigenic variation. Our findings thus suggest that the recycling of antigen variant-specific BEM cells and transport of antigen back to GC may support affinity maturation to antigenic drift.

Description
Keywords
Antigenic Drift and Shift, B-Lymphocytes, Germinal Center, Lymph Nodes, Memory B Cells
Journal Title
Nat Commun
Conference Name
Journal ISSN
2041-1723
2041-1723
Volume Title
13
Publisher
Springer Science and Business Media LLC
Sponsorship
Wellcome Trust (104384/Z/14/Z)
Medical Research Council (MR/N024907/1)
Arthritis Research UK (21777)