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Metabolic imaging with hyperpolarized [1-13C] pyruvate in patient-derived preclinical mouse models of breast cancer.

Published version
Peer-reviewed

Type

Article

Change log

Authors

Ros, Susana 
Wright, Alan J 
Bruna, Alejandra 
Brindle, Kevin M 

Abstract

13C nuclear spin hyperpolarization can increase the sensitivity of detection in an MRI experiment by more than 10,000-fold. 13C magnetic resonance spectroscopic imaging (MRSI) of hyperpolarized 13C label exchange between injected [1-13C]pyruvate and the endogenous tumor lactate pool can be used clinically to assess tumor grade and response to treatment. We describe here an experimental protocol for using this technique in patient-derived and established cell line xenograft models of breast cancer in the mouse. For complete details on the use and execution of this protocol, please refer to Ros et al. (2020).

Description

Keywords

Biophysics, Cancer, Metabolism, Model Organisms, Animals, Breast Neoplasms, Carbon Isotopes, Disease Models, Animal, Female, Heterografts, Humans, Magnetic Resonance Imaging, Mice, Pyruvic Acid

Journal Title

STAR Protoc

Conference Name

Journal ISSN

2666-1667
2666-1667

Volume Title

2

Publisher

Elsevier BV
Sponsorship
Cancer Research UK (CB4100)
Cancer Research UK (C14303/A17197)
Cancer Research Uk (None)
Cancer Research UK (unknown)
Cancer Research UK (unknown)
Cancer Research UK (unknown)
Cancer Research UK (60098573)
Cancer Research UK (unknown)
Cancer Research UK (20544)
Cancer Research UK (9401)
Cancer Research UK (16942)