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Laminin levels regulate tissue migration and anterior-posterior polarity during egg morphogenesis in Drosophila

Accepted version
Peer-reviewed

Type

Article

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Authors

Abstract

Basement membranes (BMs) are specialized extracellular matrices required for tissue organisation and organ formation. Here we study the role of laminin and its integrin receptor in the regulation of tissue migration during Drosophila oogenesis. In the fly, egg production involves the collective migration of follicle cells (FCs) over the BM to shape the mature egg. We quantify laminin and integrin levels to show that laminin contents in the BM increase with time, while integrin amounts in the FCs do not vary significantly. Controlled manipulation of integrin and laminin levels revealed that a dynamic balance of integrin-laminin amounts determines the onset and speed of FC migration. Thus, the interplay of ligand-receptor levels regulates tissue migration in vivo. In addition, reduced laminin contents affect the ultrastructure and biophysical properties of the BM. Finally, laminin depletion results also in anterior-posterior misorientation of developing follicles. Altogether, laminin emerges as a key new player in the regulation of collective cell migration, tissue stiffness and the organisation of anterior-posterior polarity in Drosophila.

Description

Keywords

31 Biological Sciences, 3105 Genetics, Generic health relevance

Journal Title

Cell Reports

Conference Name

Journal ISSN

0925-4773
1872-6356

Volume Title

145

Publisher

Elsevier
Sponsorship
Medical Research Council (G1100312)