Observations on chromosome-specific sequencing for the construction of cross-species chromosome homology maps and its resolution of human:alpaca homology.


Type
Article
Change log
Authors
Ferguson-Smith, Malcolm A 
Borges, Ana 
Kasai, Fumio 
Abstract

BACKGROUND: The history of comparative chromosome mapping is briefly reviewed, with discussion about the problem that occurs in chromosome painting when size heteromorphisms between homologues cause contamination in chromosomes sorted by flow cytometry that are used in the preparation of chromosome-specific DNA probes. MAIN BODY: As an example, we show in the alpaca (Vicagna pacos) that sequencing of contaminated chromosome sorts can reveal chromosome homologies from alignment with human and mouse genome reference sequences. The procedure identifies syntenic blocks of DNA separated in the human karyotype that are associated in the closely related alpaca and dromedary (Camelus dromedarius) karyotypes. This example provides proof of principal for the validity of the method for comparative chromosome mapping. CONCLUSION: It is suggested that the approach presented here may have application in the construction of comparative chromosome maps between distantly related taxa, such as monotremes and mammals.

Description
Keywords
Alpaca homology, Chromosome mapping, Chromosome-specific sequencing, Comparative genomics
Journal Title
Mol Cytogenet
Conference Name
Journal ISSN
1755-8166
1755-8166
Volume Title
15
Publisher
Springer Science and Business Media LLC