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CDK2 limits the highly energetic secretory program of mature β cells by restricting PEP cycle-dependent KATP channel closure.

Published version
Peer-reviewed

Type

Article

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Authors

Sdao, Sophia M 
Ho, Thuong 
Foster, Hannah R 
De Leon, Elizabeth R 

Abstract

Hallmarks of mature β cells are restricted proliferation and a highly energetic secretory state. Paradoxically, cyclin-dependent kinase 2 (CDK2) is synthesized throughout adulthood, its cytosolic localization raising the likelihood of cell cycle-independent functions. In the absence of any changes in β cell mass, maturity, or proliferation, genetic deletion of Cdk2 in adult β cells enhanced insulin secretion from isolated islets and improved glucose tolerance in vivo. At the single β cell level, CDK2 restricts insulin secretion by increasing KATP conductance, raising the set point for membrane depolarization in response to activation of the phosphoenolpyruvate (PEP) cycle with mitochondrial fuels. In parallel with reduced β cell recruitment, CDK2 restricts oxidative glucose metabolism while promoting glucose-dependent amplification of insulin secretion. This study provides evidence of essential, non-canonical functions of CDK2 in the secretory pathways of quiescent β cells.

Description

Keywords

CDK2, K(ATP) channel, PEP cycle, amplifying pathways, biosensor imaging, calcium, electrophysiology, insulin secretion, metabolic oscillations, β cell metabolism, Animals, B-Lymphocytes, Cyclin-Dependent Kinase 2, Humans, KATP Channels, Mice

Journal Title

Cell Rep

Conference Name

Journal ISSN

2211-1247
2211-1247

Volume Title

34

Publisher

Elsevier BV