1H, 15N and 13C resonance assignments of the HR1c domain of PRK1, a protein kinase C-related kinase.


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Authors
Sophocleous, Georgios 
Wood, George 
Abstract

PRK1 is a member of the protein kinase C-related kinase (PRK) family of serine/threonine kinases and a downstream effector of Rho GTPases. PRK1 has three N-terminal Homology Region 1 (HR1) domains (HR1a, HR1b and HR1c), which form antiparallel coiled coils that interact with Rho family GTPases. PRK1 also has a C2-like domain that targets it to the plasma membrane and a kinase domain, which is a member of the protein kinase C superfamily. PRK1 is involved in cytoskeletal regulation, cell adhesion, cell cycle progression and the immune response, and is implicated in cancer. There is currently no structural information for the HR1c domain. The 1H, 15N and 13C NMR backbone and sidechain resonance assignment of the HR1c domain presented here forms the basis for this domain's structural characterisation. This work will also enable studies of interactions between the three HR1 domains in an effort to obtain structural insight into the regulation of PRK1 activity.

Description

Funder: A.G. Levintis Foundation

Keywords
Coiled-coil, HR1 domain, PKN, Protein Kinase C related kinase, Small GTPase, Amino Acid Sequence, Carbon-13 Magnetic Resonance Spectroscopy, Humans, Nitrogen Isotopes, Protein Domains, Protein Kinase C, Protein Structure, Secondary, Proton Magnetic Resonance Spectroscopy
Journal Title
Biomol NMR Assign
Conference Name
Journal ISSN
1874-2718
1874-270X
Volume Title
14
Publisher
Springer Science and Business Media LLC