KIXBASE: A comprehensive web resource for identification and exploration of KIX domains.

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cam.issuedOnline2017-11-02
dc.contributor.authorYadav, Archana
dc.contributor.authorThakur, Jitendra K
dc.contributor.authorYadav, Gitanjali
dc.contributor.orcidYadav, Gitanjali [0000-0001-6591-9964]
dc.date.accessioned2018-09-11T17:32:15Z
dc.date.available2018-09-11T17:32:15Z
dc.date.issued2017-11-02
dc.description.abstractThe KIX domain has emerged in the last two decades as a critical site of interaction for transcriptional assembly, regulation and gene expression. Discovered in 1994, this conserved, triple helical globular domain has been characterised in various coactivator proteins of yeast, mammals and plants, including the p300/CBP (a histone acetyl transferase), MED15 (a subunit of the mediator complex of RNA polymerase II), and RECQL5 helicases. In this work, we describe the first rigorous meta analysis of KIX domains across all forms of life, leading to the development of KIXBASE, a predictive web server and global repository for detection and analysis of KIX domains. To our knowledge, KIXBASE comprises the largest online collection of KIX sequences, enabling assessments at the level of both sequence and structure, incorporating PSIPRED and MUSTER at the backend for further annotation and quality assessment. In addition, KIXBASE provides useful information about critical aspects of KIX domains such as their intrinsic disorder, hydrophobicity profiles, functional classification and annotation based on domain architectures. KIXBASE represents a significant enrichment of the currently annotated KIX dataset, especially in the plant kingdom, thus highlighting potential targets for biochemical characterization. The KIX webserver and database are both freely available to the scientific community, at http://www.nipgr.res.in/kixbase/home.php .
dc.format.mediumElectronic
dc.identifier.doi10.17863/CAM.27562
dc.identifier.eissn2045-2322
dc.identifier.issn2045-2322
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/280195
dc.languageeng
dc.publisherSpringer Science and Business Media LLC
dc.publisher.urlhttp://dx.doi.org/10.1038/s41598-017-14617-0
dc.rightsAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectAmino Acid Sequence
dc.subjectAnimals
dc.subjectHumans
dc.subjectInternet
dc.subjectMediator Complex
dc.subjectModels, Molecular
dc.subjectProtein Domains
dc.subjectRecQ Helicases
dc.subjectSequence Alignment
dc.subjectSoftware
dc.subjectp300-CBP Transcription Factors
dc.titleKIXBASE: A comprehensive web resource for identification and exploration of KIX domains.
dc.typeArticle
dcterms.dateAccepted2017-10-10
prism.issueIdentifier1
prism.publicationDate2017
prism.publicationNameSci Rep
prism.startingPage14924
prism.volume7
rioxxterms.licenseref.startdate2017-11-02
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.typeJournal Article/Review
rioxxterms.versionofrecord10.1038/s41598-017-14617-0
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