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Transposon-driven transcription is a conserved feature of vertebrate spermatogenesis and transcript evolution.

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cam.issuedOnline2017-05-12
dc.contributor.authorDavis, Matthew P
dc.contributor.authorCarrieri, Claudia
dc.contributor.authorSaini, Harpreet K
dc.contributor.authorvan Dongen, Stijn
dc.contributor.authorLeonardi, Tommaso
dc.contributor.authorBussotti, Giovanni
dc.contributor.authorMonahan, Jack M
dc.contributor.authorAuchynnikava, Tania
dc.contributor.authorBitetti, Angelo
dc.contributor.authorRappsilber, Juri
dc.contributor.authorAllshire, Robin C
dc.contributor.authorShkumatava, Alena
dc.contributor.authorO'Carroll, Dónal
dc.contributor.authorEnright, Anton J
dc.contributor.orcidRappsilber, Juri [0000-0001-5999-1310]
dc.contributor.orcidO'Carroll, Dónal [0000-0002-8626-2217]
dc.contributor.orcidEnright, Anton J [0000-0002-6090-3100]
dc.date.accessioned2017-11-23T15:13:05Z
dc.date.available2017-11-23T15:13:05Z
dc.date.issued2017-07
dc.description.abstractSpermatogenesis is associated with major and unique changes to chromosomes and chromatin. Here, we sought to understand the impact of these changes on spermatogenic transcriptomes. We show that long terminal repeats (LTRs) of specific mouse endogenous retroviruses (ERVs) drive the expression of many long non-coding transcripts (lncRNA). This process occurs post-mitotically predominantly in spermatocytes and round spermatids. We demonstrate that this transposon-driven lncRNA expression is a conserved feature of vertebrate spermatogenesis. We propose that transposon promoters are a mechanism by which the genome can explore novel transcriptional substrates, increasing evolutionary plasticity and allowing for the genesis of novel coding and non-coding genes. Accordingly, we show that a small fraction of these novel ERV-driven transcripts encode short open reading frames that produce detectable peptides. Finally, we find that distinct ERV elements from the same subfamilies act as differentially activated promoters in a tissue-specific context. In summary, we demonstrate that LTRs can act as tissue-specific promoters and contribute to post-mitotic spermatogenic transcriptome diversity.
dc.format.mediumPrint-Electronic
dc.identifier.doi10.17863/CAM.15838
dc.identifier.eissn1469-3178
dc.identifier.issn1469-221X
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/269628
dc.languageeng
dc.language.isoeng
dc.publisherSpringer Science and Business Media LLC
dc.publisher.urlhttp://dx.doi.org/10.15252/embr.201744059
dc.rightsAttribution 4.0 International
dc.rightsAttribution 4.0 International
dc.rightsAttribution 4.0 International
dc.rightsAttribution 4.0 International
dc.rightsAttribution 4.0 International
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectendogenous retroviruses
dc.subjectgenome evolution
dc.subjectlncRNA
dc.subjectspermatogenesis
dc.subjecttranscriptome
dc.subjectAnimals
dc.subjectDNA Transposable Elements
dc.subjectEndogenous Retroviruses
dc.subjectEvolution, Molecular
dc.subjectGenomics
dc.subjectMale
dc.subjectMice
dc.subjectOpen Reading Frames
dc.subjectPromoter Regions, Genetic
dc.subjectRNA, Long Noncoding
dc.subjectSpermatocytes
dc.subjectSpermatogenesis
dc.subjectTerminal Repeat Sequences
dc.subjectTranscription, Genetic
dc.subjectTranscriptome
dc.titleTransposon-driven transcription is a conserved feature of vertebrate spermatogenesis and transcript evolution.
dc.typeArticle
dcterms.dateAccepted2017-04-11
prism.endingPage1247
prism.issueIdentifier7
prism.publicationDate2017
prism.publicationNameEMBO Rep
prism.startingPage1231
prism.volume18
rioxxterms.licenseref.startdate2017-07
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.typeJournal Article/Review
rioxxterms.versionVoR
rioxxterms.versionofrecord10.15252/embr.201744059

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