Proteomic plasma membrane profiling reveals an essential role for gp96 in the cell surface expression of LDLR family members, including the LDL receptor and LRP6.

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Weekes, Michael P 
Antrobus, Robin 
Talbot, Suzanne 
Hör, Simon 
Simecek, Nikol 

The endoplasmic reticulum chaperone gp96 is required for the cell surface expression of a narrow range of proteins, including toll-like receptors (TLRs) and integrins. To identify a more comprehensive repertoire of proteins whose cell surface expression is dependent on gp96, we developed plasma membrane profiling (PMP), a technique that combines SILAC labeling with selective cell surface aminooxy-biotinylation. This approach allowed us to compare the relative abundance of plasma membrane (PM) proteins on gp96-deficient versus gp96-reconstituted murine pre-B cells. Analysis of unfractionated tryptic peptides initially identified 113 PM proteins, which extended to 706 PM proteins using peptide prefractionation. We confirmed a requirement for gp96 in the cell surface expression of certain TLRs and integrins and found a marked decrease in cell surface expression of four members of the extended LDL receptor family (LDLR, LRP6, Sorl1 and LRP8) in the absence of gp96. Other novel gp96 client proteins included CD180/Ly86, important in the B-cell response to lipopolysaccharide. We highlight common structural motifs in these client proteins that may be recognized by gp96, including the beta-propeller and leucine-rich repeat. This study therefore identifies the extended LDL receptor family as an important new family of proteins whose cell surface expression is regulated by gp96.

Animals, Antigens, CD, Cell Line, Cell Membrane, Chromatography, High Pressure Liquid, Down-Regulation, Integrins, Low Density Lipoprotein Receptor-Related Protein-6, Membrane Glycoproteins, Membrane Proteins, Mice, Peptide Fragments, Protein Interaction Mapping, Proteomics, Receptors, LDL, Tandem Mass Spectrometry, Toll-Like Receptors
Journal Title
J Proteome Res
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American Chemical Society (ACS)
Medical Research Council (G9800943)
Wellcome Trust (084957/Z/08/Z)
Wellcome Trust (086615/Z/08/Z)