Repository logo
 

The cytotoxic effect of TiF4 and NaF on fibroblasts is influenced by the experimental model, fluoride concentration and exposure time.

Simple item page
cam.issuedOnline2017-06-14
dc.contributor.authorSalomão, Priscila Maria Aranda
dc.contributor.authorOliveira, Flávia Amadeu de
dc.contributor.authorRodrigues, Paula Danielle
dc.contributor.authorAl-Ahj, Luana Polioni
dc.contributor.authorGasque, Kellen Cristina da Silva
dc.contributor.authorJeggle, Pia
dc.contributor.authorBuzalaf, Marilia Afonso Rabelo
dc.contributor.authorOliveira, Rodrigo Cardoso de
dc.contributor.authorEdwardson, John Michael
dc.contributor.authorMagalhães, Ana Carolina
dc.contributor.orcidMagalhães, Ana Carolina [0000-0002-6413-5348]
dc.date.accessioned2018-10-22T06:54:06Z
dc.date.available2018-10-22T06:54:06Z
dc.date.issued2017
dc.description.abstractOBJECTIVE: Titanium tetrafluoride (TiF4) has shown promising effect in preventing tooth lesions. Therefore, we compared the cytotoxicity of TiF4 with sodium fluoride (NaF) (already applied in Dentistry) considering different fluoride concentrations, pH values and experimental models. MATERIALS AND METHODS: Step 1) NIH/3T3 fibroblasts were exposed to mediums containing NaF or TiF4 (from 0.15 to 2.45% F), both at native and adjusted pH, for 6 h. Step 2) NIH/3T3 were exposed to NaF or TiF4 varnishes with 0.95, 1.95 or 2.45% F (native pH), for 6, 12 or 24 h. We applied MTT (1st and 2nd steps) and Hoescht/PI stain (2nd step) assays. Step 3) NIH/3T3 were exposed to NaF or TiF4 varnish (2.45% F), at native pH, for 6 or 12 h. The cell stiffness was measured by atomic force microscopy (AFM). RESULTS: Step 1) All cells exposed to NaF or TiF4 mediums died, regardless of the F concentration and pH. Step 2) Both varnishes, at 1.90 and 2.45% F, reduced cell viability by similar extents (33-86% at 6 h, 35-93% at 12 h, and 87-98% at 24 h) compared with control, regardless of the type of fluoride. Varnishes with 0.95% F did not differ from control. Step 3) TiF4 and NaF reduced cell stiffness to a similar extent, but only TiF4 differed from control at 6 h. CONCLUSIONS: Based on the results of the 3 experimental steps, we conclude that TiF4 and NaF have similar cytotoxicity. The cytotoxicity was dependent on F concentration and exposure time. This result gives support for testing the effect of TiF4 varnish in vivo.
dc.format.mediumElectronic-eCollection
dc.identifier.doi10.17863/CAM.31562
dc.identifier.eissn1932-6203
dc.identifier.issn1932-6203
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/284194
dc.languageeng
dc.language.isoeng
dc.publisherPublic Library of Science (PLoS)
dc.publisher.urlhttp://dx.doi.org/10.1371/journal.pone.0179471
dc.rightsAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectAnimals
dc.subjectCariostatic Agents
dc.subjectCell Physiological Phenomena
dc.subjectCell Survival
dc.subjectDose-Response Relationship, Drug
dc.subjectFibroblasts
dc.subjectFluorides
dc.subjectHydrogen-Ion Concentration
dc.subjectMice
dc.subjectMicroscopy, Atomic Force
dc.subjectModels, Theoretical
dc.subjectNIH 3T3 Cells
dc.subjectSodium Fluoride
dc.subjectTime Factors
dc.subjectTitanium
dc.titleThe cytotoxic effect of TiF4 and NaF on fibroblasts is influenced by the experimental model, fluoride concentration and exposure time.
dc.typeArticle
dcterms.dateAccepted2017-05-31
prism.issueIdentifier6
prism.publicationDate2017
prism.publicationNamePLoS One
prism.startingPagee0179471
prism.volume12
rioxxterms.licenseref.startdate2017-01
rioxxterms.licenseref.urihttp://creativecommons.org/licenses/by/4.0/
rioxxterms.typeJournal Article/Review
rioxxterms.versionVoR
rioxxterms.versionofrecord10.1371/journal.pone.0179471

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
The cytotoxic effect of TiF4 and NaF on fibroblasts is influenced by the experimental model, fluoride concentration and exposure time.pdf
Size:
14.17 MB
Format:
Adobe Portable Document Format
Description:
Published version
Licence
https://creativecommons.org/licenses/by/4.0/
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
DepositLicenceAgreement.pdf
Size:
417.78 KB
Format:
Adobe Portable Document Format
Download

Collections

Cambridge University Research Outputs