N-methyl-D-aspartate receptors mediate the phosphorylation and desensitization of muscarinic receptors in cerebellar granule neurons.

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Butcher, Adrian J 
Torrecilla, Ignacio 
Young, Kenneth W 
Kong, Kok Choi 
Mistry, Sharad C 

Changes in synaptic strength mediated by ionotropic glutamate N-methyl-D-asparate (NMDA) receptors is generally considered to be the molecular mechanism underlying memory and learning. NMDA receptors themselves are subject to regulation through signaling pathways that are activated by G-protein-coupled receptors (GPCRs). In this study we investigate the ability of NMDA receptors to regulate the signaling of GPCRs by focusing on the G(q/11)-coupled M(3)-muscarinic receptor expressed endogenously in mouse cerebellar granule neurons. We show that NMDA receptor activation results in the phosphorylation and desensitization of M(3)-muscarinic receptors through a mechanism dependent on NMDA-mediated calcium influx and the activity of calcium-calmodulin-dependent protein kinase II. Our study reveals a complex pattern of regulation where GPCRs (M(3)-muscarinic) and NMDA receptors can feedback on each other in a process that is likely to influence the threshold value of signaling networks involved in synaptic plasticity.

Amino Acid Sequence, Animals, Calcium Signaling, Calcium-Calmodulin-Dependent Protein Kinase Type 2, Cells, Cultured, Cerebellum, Feedback, Physiological, Mice, Mice, Knockout, Molecular Sequence Data, N-Methylaspartate, Neuronal Plasticity, Neurons, Phosphatidylinositols, Phosphorylation, Receptor, Muscarinic M3, Receptors, N-Methyl-D-Aspartate, Recombinant Fusion Proteins, Signal Transduction
Journal Title
J Biol Chem
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Elsevier BV