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Evaluation of T cell responses to naturally processed variant SARS-CoV-2 spike antigens in individuals following infection or vaccination.

Published version
Peer-reviewed

Repository DOI


Type

Article

Change log

Authors

Yin, Zixi 
Chen, Ji-Li 
Lu, Yongxu 
Wang, Beibei 
Godfrey, Leila 

Abstract

Most existing studies characterizing severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)-specific T cell responses are peptide based. This does not allow evaluation of whether tested peptides are processed and presented canonically. In this study, we use recombinant vaccinia virus (rVACV)-mediated expression of SARS-CoV-2 spike protein and SARS-CoV-2 infection of angiotensin-converting enzyme (ACE)-2-transduced B cell lines to evaluate overall T cell responses in a small cohort of recovered COVID-19 patients and uninfected donors vaccinated with ChAdOx1 nCoV-19. We show that rVACV expression of SARS-CoV-2 antigen can be used as an alternative to SARS-CoV-2 infection to evaluate T cell responses to naturally processed spike antigens. In addition, the rVACV system can be used to evaluate the cross-reactivity of memory T cells to variants of concern (VOCs) and to identify epitope escape mutants. Finally, our data show that both natural infection and vaccination could induce multi-functional T cell responses with overall T cell responses remaining despite the identification of escape mutations.

Description

Keywords

CP: Immunology, ChAdOx1 nCoV-19, SARS-CoV-2, T cell, antigen processing and presentation, immune escape, recombinant vaccinia virus, spike protein, variants of concern, Humans, SARS-CoV-2, COVID-19, ChAdOx1 nCoV-19, Vaccination, Antibodies, Viral

Journal Title

Cell Rep

Conference Name

Journal ISSN

2211-1247
2211-1247

Volume Title

42

Publisher

Elsevier BV
Sponsorship
Wellcome Trust (090315/Z/09/Z)