RNAseq Kiss1 Neurons
Repository URI
Repository DOI
Type
Dataset
Change log
Authors
Colledge, William
Description
Pools of 10 - 15 Kiss1 neurons from the rostral (R samples) or caudal (C samples) regions of the arcuate or the RP3V region (A samples) were transferred to a separate PCR tube containing a 8 μl of water, 1 μl 0.1mM DTT and 1 μl RNase inhibitor (40U RNase OUT, Invitrogen) and snap-frozen on dry ice and kept at -80⸰C until further analysis.
Library preparation was performed using SMART-Seq v4 Ultra Low Input RNA Kit (Takara Bio USA, Inc. Cat#: 634889).
Samples were sequenced induplicate using single-end 75 bp rreads on a NextSeq500 (Illumina, USA) machine using NextSeq 500/550 High Output v2 Kit (75 cycles) (Illumina, USA). The sequencing depth of the raw reads ranged from 23-49 million per sample.
Version
Software / Usage instructions
Each biological sample was sequences twice (experimental replicates). These have the same number (eg A4, C6 etc). These fastq files can be combined before bioinformatic analysis.
The fastq files can be viewed in a text editor.
Keywords
hypothalamaus, Kiss1, RNAseq
Publisher
Sponsorship
This work was funded by a British Council Commonwealth Scholarship and the Ford Physiology Fund Endowment, University of Cambridge.