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Widespread transposon co-option in the Caenorhabditis germline regulatory network.

Published version
Peer-reviewed

Type

Article

Change log

Authors

Carelli, Francesco Nicola  ORCID logo  https://orcid.org/0000-0003-4280-4923
Cerrato, Chiara 
Dong, Yan 
Appert, Alex 

Abstract

The movement of selfish DNA elements can lead to widespread genomic alterations with potential to create novel functions. We show that transposon expansions in Caenorhabditis nematodes led to extensive rewiring of germline transcriptional regulation. We find that about one-third of Caenorhabditis elegans germline-specific promoters have been co-opted from two related miniature inverted repeat transposable elements (TEs), CERP2 and CELE2. These promoters are regulated by HIM-17, a THAP domain-containing transcription factor related to a transposase. Expansion of CERP2 occurred before radiation of the Caenorhabditis genus, as did fixation of mutations in HIM-17 through positive selection, whereas CELE2 expanded only in C. elegans. Through comparative analyses in Caenorhabditis briggsae, we find not only evolutionary conservation of most CERP2 co-opted promoters but also a substantial fraction that are species-specific. Our work reveals the emergence and evolutionary conservation of a novel transcriptional network driven by TE co-option with a major impact on regulatory evolution.

Description

Keywords

31 Biological Sciences, 3102 Bioinformatics and Computational Biology, 3105 Genetics, Genetics, Human Genome, Generic health relevance

Journal Title

Sci Adv

Conference Name

Journal ISSN

2375-2548
2375-2548

Volume Title

8

Publisher

American Association for the Advancement of Science (AAAS)
Sponsorship
Wellcome Trust (101863/Z/13/Z)
Wellcome Trust (203144/A/16/Z)
Wellcome Trust (217170/Z/19/Z)
Wellcome Trust (203144/Z/16/Z)
Wellcome Trust (092096/Z/10/Z)
Cancer Research Uk (None)
MRC (MR/S021620/1)
Welcome Senior Research Fellowship 101863 (JA), Wellcome Investigator award 217170 (JA) Wellcome core grant 092096 (Gurdon Institute) CRUK core grant C6946/A14492 (Gurdon Institute) Swiss National Science Foundation Postdoc Mobility Fellowship P400PB_180795 (FNC) EMBO Long-Term Fellowship ALTF 936-2017 (FNC)