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Phosphate Deprivation Can Impair Mechano-Stimulated Cytosolic Free Calcium Elevation in Arabidopsis Roots.

Published version
Peer-reviewed

Type

Article

Change log

Authors

Matthus, Elsa 
Doddrell, Nicholas H  ORCID logo  https://orcid.org/0000-0002-6567-2401
Guillaume, Gaëtan 
Mohammad-Sidik, Amirah B  ORCID logo  https://orcid.org/0000-0002-5375-9489

Abstract

The root tip responds to mechanical stimulation with a transient increase in cytosolic free calcium as a possible second messenger. Although the root tip will grow through a heterogeneous soil nutrient supply, little is known of the consequence of nutrient deprivation for such signalling. Here, the effect of inorganic phosphate deprivation on the root's mechano-stimulated cytosolic free calcium increase is investigated. Arabidopsisthaliana (cytosolically expressing aequorin as a bioluminescent free calcium reporter) is grown in zero or full phosphate conditions, then roots or root tips are mechanically stimulated. Plants also are grown vertically on a solid medium so their root skewing angle (deviation from vertical) can be determined as an output of mechanical stimulation. Phosphate starvation results in significantly impaired cytosolic free calcium elevation in both root tips and whole excised roots. Phosphate-starved roots sustain a significantly lower root skewing angle than phosphate-replete roots. These results suggest that phosphate starvation causes a dampening of the root mechano-signalling system that could have consequences for growth in hardened, compacted soils.

Description

Keywords

Arabidopsis, FERONIA, calcium, mechano, phosphate, root, skew, touch

Journal Title

Plants (Basel)

Conference Name

Journal ISSN

2223-7747
2223-7747

Volume Title

9

Publisher

MDPI AG
Sponsorship
Biotechnology and Biological Sciences Research Council (BB/J014540/1)