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User-friendly oblique plane microscopy on a fully functional commercially available microscope base.

Accepted version
Peer-reviewed

Type

Article

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Authors

Nashchekin, Dmitry 
St Johnston, Daniel 

Abstract

In this work we present an oblique plane microscope designed to work seamlessly with a commercially available microscope base. To support all the functionality offered by the microscope base, where the position of the objective lens is not fixed, we adopted a two-mirror scanning geometry that can compensate for changes to the position of the objective lens during routine microscope operation. We showed that within a ± 1 mm displacement range of the 100X, 1.35 NA objective lens away from its designed position, the PSF size increased by <3% and <11% in the lateral and axial dimensions, respectively, while the error in magnification was <0.5% within volumes extending ± 10 µm about the focal plane. Compared to the more traditional scan-lens/galvo-mirror combination, the two-mirror scanning geometry offers higher light efficiency and a more compact footprint, which could be beneficial to all OPM designs regardless of the use of a commercial base or not.

Description

Keywords

32 Biomedical and Clinical Sciences, 3212 Ophthalmology and Optometry

Journal Title

Biomed Opt Express

Conference Name

Journal ISSN

2156-7085
2156-7085

Volume Title

Publisher

Optica Publishing Group
Sponsorship
Biotechnology and Biological Sciences Research Council (BB/P026486/1)
Wellcome Trust (080007/Z/06/Z)
Cancer Research UK (C6946/A24843)
Wellcome Trust (207496/Z/17/Z)
Wellcome Trust (203144/A/16/Z)
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