Determination of complex subclonal structures of hematological malignancies by multiplexed genotyping of blood progenitor colonies.
Published version
Peer-reviewed
Repository URI
Repository DOI
Change log
Authors
Abstract
Current next-generation sequencing (NGS) technologies allow unprecedented insights into the mutational profiles of tumors. Recent studies in myeloproliferative neoplasms have further demonstrated that, not only the mutational profile, but also the order in which these mutations are acquired is relevant for our understanding of the disease. Our ability to assign mutation order from NGS data alone is, however, limited. Here, we present a strategy of highly multiplexed genotyping of burst forming unit-erythroid colonies based on NGS results to assess subclonal tumor structure. This allowed for the generation of complex clonal hierarchies and determination of order of mutation acquisition far more accurately than was possible from NGS data alone.
Description
Keywords
Journal Title
Conference Name
Journal ISSN
1873-2399
Volume Title
Publisher
Publisher DOI
Sponsorship
Wellcome Trust (100140/Z/12/Z)
Wellcome Trust (097922/Z/11/Z)
Wellcome Trust (104710/Z/14/Z)
Cancer Research UK (21762)
Leukemia & Lymphoma Society (7001-12)
Leukaemia & Lymphoma Research (13003)
Cancer Research Uk (None)