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HIV-1 Packaging Visualised by In-Gel SHAPE.

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cam.issuedOnline2021-11-29
dc.contributor.authorD'Souza, Aaron R
dc.contributor.authorJayaraman, Dhivya
dc.contributor.authorLong, Ziqi
dc.contributor.authorZeng, Jingwei
dc.contributor.authorPrestwood, Liam J
dc.contributor.authorChan, Charlene
dc.contributor.authorKappei, Dennis
dc.contributor.authorLever, Andrew
dc.contributor.authorKenyon, Julia
dc.contributor.orcidLong, Ziqi [0000-0002-4666-9936]
dc.contributor.orcidZeng, Jingwei [0000-0002-9802-6019]
dc.contributor.orcidKappei, Dennis [0000-0002-3582-2253]
dc.contributor.orcidKenyon, Julia [0000-0001-6055-7052]
dc.date.accessioned2021-12-22T15:06:46Z
dc.date.available2021-12-22T15:06:46Z
dc.date.issued2021-11-29
dc.date.updated2021-12-22T15:06:45Z
dc.description.abstractHIV-1 packages two copies of its gRNA into virions via an interaction with the viral structural protein Gag. Both copies and their native RNA structure are essential for virion infectivity. The precise stepwise nature of the packaging process has not been resolved. This is largely due to a prior lack of structural techniques that follow RNA structural changes within an RNA-protein complex. Here, we apply the in-gel SHAPE (selective 2'OH acylation analysed by primer extension) technique to study the initiation of HIV-1 packaging, examining the interaction between the packaging signal RNA and the Gag polyprotein, and compare it with that of the NC domain of Gag alone. Our results imply interactions between Gag and monomeric packaging signal RNA in switching the RNA conformation into a dimerisation-competent structure, and show that the Gag-dimer complex then continues to stabilise. These data provide a novel insight into how HIV-1 regulates the translation and packaging of its genome.
dc.description.sponsorshipThis work was funded by an Eric Reid fund for Methodology grant from the Biochemical Society (to JCK), UK Medical Research Council, Grant/Award Number: MR/N022939/1 (to AML and JCK). AMLL is supported by the Clinical Academic Reserve and his laboratory by the NIHR Cambridge BRC (Grant RCAG/18). Research in the Kappei laboratory was supported by the National Research Foundation Singapore and the Singapore Ministry of Education under its Re-search Centres of Excellence initiative.
dc.identifier.doi10.17863/CAM.79153
dc.identifier.eissn1999-4915
dc.identifier.issn1999-4915
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/331703
dc.languageen
dc.publisherMDPI AG
dc.subjectHIV-1
dc.subjectpackaging
dc.subjectin-gel SHAPE
dc.subjectGag
dc.subjectNC
dc.subjectRNA structure
dc.subjectdimerisation
dc.titleHIV-1 Packaging Visualised by In-Gel SHAPE.
dc.typeArticle
dcterms.dateAccepted2021-11-23
prism.issueIdentifier12
prism.publicationNameViruses
prism.volume13
pubs.funder-project-idMedical Research Council (MR/N022939/1)
rioxxterms.licenseref.urihttps://creativecommons.org/licenses/by/4.0/
rioxxterms.versionVoR
rioxxterms.versionofrecord10.3390/v13122389

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