cam.issuedOnline	2022-06-25
dc.contributor.author	Desjardins, Stuart D
dc.contributor.author	Simmonds, James
dc.contributor.author	Guterman, Inna
dc.contributor.author	Kanyuka, Kostya
dc.contributor.author	Burridge, Amanda J
dc.contributor.author	Tock, Andrew
dc.contributor.author	Sanchez-Moran, Eugenio
dc.contributor.author	Franklin, F Chris H
dc.contributor.author	Henderson, Ian
dc.contributor.author	Edwards, Keith J
dc.contributor.author	Uauy, Cristobal
dc.contributor.author	Higgins, James D
dc.contributor.orcid	Simmonds, James [0000-0003-4936-1694]
dc.contributor.orcid	Kanyuka, Kostya [0000-0001-6324-4123]
dc.contributor.orcid	Burridge, Amanda J [0000-0002-0140-5523]
dc.contributor.orcid	Tock, Andrew [0000-0002-6590-8314]
dc.contributor.orcid	Henderson, Ian [0000-0001-5066-1489]
dc.contributor.orcid	Uauy, Cristobal [0000-0002-9814-1770]
dc.contributor.orcid	Higgins, James D [0000-0001-6027-8678]
dc.date.accessioned	2022-06-29T19:46:48Z
dc.date.available	2022-06-29T19:46:48Z
dc.date.issued	2022-06-25
dc.date.submitted	2022-02-22
dc.date.updated	2022-06-29T19:46:48Z
dc.description.abstract	FANCM suppresses crossovers in plants by unwinding recombination intermediates. In wheat, crossovers are skewed toward the chromosome ends, thus limiting generation of novel allelic combinations. Here, we observe that FANCM maintains the obligate crossover in tetraploid and hexaploid wheat, thus ensuring that every chromosome pair exhibits at least one crossover, by localizing class I crossover protein HEI10 at pachytene. FANCM also suppresses class II crossovers that increased 2.6-fold in fancm msh5 quadruple mutants. These data are consistent with a role for FANCM in second-end capture of class I designated crossover sites, whilst FANCM is also required to promote formation of non-crossovers. In hexaploid wheat, genetic mapping reveals that crossovers increase by 31% in fancm compared to wild type, indicating that fancm could be an effective tool to accelerate breeding. Crossover rate differences in fancm correlate with wild type crossover distributions, suggesting that chromatin may influence the recombination landscape in similar ways in both wild type and fancm.
dc.identifier.doi	10.17863/CAM.85939
dc.identifier.eissn	2041-1723
dc.identifier.issn	2041-1723
dc.identifier.other	s41467-022-31438-6
dc.identifier.other	31438
dc.identifier.uri	https://www.repository.cam.ac.uk/handle/1810/338526
dc.language	en
dc.publisher	Springer Science and Business Media LLC
dc.subject	Article
dc.subject	/631/208/1405
dc.subject	/631/80/641/1633
dc.subject	/631/449/2491
dc.subject	/14/32
dc.subject	/14/63
dc.subject	/45
dc.subject	/45/77
dc.subject	article
dc.title	FANCM promotes class I interfering crossovers and suppresses class II non-interfering crossovers in wheat meiosis.
dc.type	Article
dcterms.dateAccepted	2022-06-17
prism.issueIdentifier	1
prism.publicationName	Nat Commun
prism.volume	13
pubs.funder-project-id	RCUK \| Biotechnology and Biological Sciences Research Council (BBSRC) (BB/M014908/1)
rioxxterms.licenseref.uri	http://creativecommons.org/licenses/by/4.0/
rioxxterms.version	VoR
rioxxterms.versionofrecord	10.1038/s41467-022-31438-6

FANCM promotes class I interfering crossovers and suppresses class II non-interfering crossovers in wheat meiosis.

Files

Original bundle

Collections