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Evaluating the Sensitivity and Specificity of Siemens Clinitest Lateral Flow Test and the Simple AMplification-Based Assay (SAMBA)-2 PCR Test for SARS-CoV-2 Infection.

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cam.issuedOnline2021-09-27
dc.contributor.authorBoyle, Adrian A
dc.contributor.authorHardwick, Susie
dc.contributor.authorWarne, Ben
dc.contributor.authorNwuba, Chukwuneyem Kosisochukwu
dc.contributor.authorBrown, Nicholas
dc.contributor.authorShaw, Ashley
dc.contributor.orcidWarne, Ben [0000-0003-1326-0373]
dc.date.accessioned2022-01-06T12:55:49Z
dc.date.available2022-01-06T12:55:49Z
dc.date.issued2021-09
dc.date.updated2022-01-06T12:55:48Z
dc.description.abstractIntroduction Accurate point-of-care testing for SARS-CoV-2 could quickly identify which patients need to be isolated and improve flow for patients being admitted as an emergency to the hospital. We evaluated two diagnostic tests with shorter turnaround times, the Siemens Clinitest Lateral Flow (Siemens Healthineers AG, Erlangen, Germany) and the Simple AMplification-Based Assay (SAMBA)-2 PCR test against a standard laboratory PCR test. Methods We conducted a prospective diagnostic cohort study in a single English emergency department. Adult participants underwent three swabs: the Siemens Clinitest Lateral Flow Test, the SAMBA-2 and a standard laboratory PCR test. Results A total of 212 participants were recruited. The sensitivity and specificity of the Siemens Clinitest Lateral Flow Test against the laboratory PCR test was 55.6% (95% CI 30.8-78.5) and 100% (95% CI 98.1-100) respectively. The sensitivity and specificity of the SAMBA-2 PCR test against the laboratory PCR test was 60.0% (95% CI 32.3-83.7) and 100% (95% CI 97.9-100) respectively. Conclusion Neither the Siemens Clinitest Lateral Flow Test nor the SAMBA-2 PCR test demonstrated sufficient sensitivity to rule out active SARS-CoV-2 infection. Both tests demonstrated high specificity.
dc.identifier.doi10.17863/CAM.79659
dc.identifier.eissn2168-8184
dc.identifier.issn2168-8184
dc.identifier.otherPMC8553283
dc.identifier.other34725589
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/332213
dc.languageeng
dc.language.isoeng
dc.publisherSpringer Science and Business Media LLC
dc.publisher.urlhttp://dx.doi.org/10.7759/cureus.18319
dc.rightsAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.sourceessn: 2168-8184
dc.sourcenlmid: 101596737
dc.subjectcovid-19
dc.subjectemergency department
dc.subjectpcr test
dc.subjectsamba-2
dc.subjectsiemens clinitest
dc.titleEvaluating the Sensitivity and Specificity of Siemens Clinitest Lateral Flow Test and the Simple AMplification-Based Assay (SAMBA)-2 PCR Test for SARS-CoV-2 Infection.
dc.typeArticle
dcterms.dateAccepted2021-09-27
prism.issueIdentifier9
prism.publicationNameCureus
prism.volume13
rioxxterms.licenseref.urihttps://creativecommons.org/licenses/by/4.0/
rioxxterms.versionVoR
rioxxterms.versionofrecord10.7759/cureus.18319

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