This dataset corresponds to the final figures from ‘GIBBERELLIN PERCEPTION SENSOR 2 reveals genesis and role of cellular GA dynamics in light-regulated hypocotyl growth’ by Jayne Griffiths, Annalisa Rizza, Bijun Tang, Wolf B. Fromer and Alexander M. Jones. Data was collected at Sainsbury Laboratory, Cambridge University between approximately April 2019 and October 2023. Any queries on the data can be emailed to Alexander M Jones (alexander.jones@slcu.cam.ac.uk), the principal investigator on the project, or Jayne Griffiths (jayne.griffiths@slcu.cam.ac.uk), the lead author. Notes on the dataset: All data is placed under a CC-BY-NC 4.0 International license. Methods are detailed fully in the paper but include: Confocal microscopy, Advanced image analysis, Arabidopsis hypocotyl phenotyping, RT-qPCR. Microscopy data was analysed with Fiji and FRETENATOR1.5 as detailed in the paper. Due to size constraints we have been unable to include all of our analysis but we have tried to include original image files as lif or tif files with excel files containing processed data. The metadata file includes the settings that were used to process the images using the FRETENATOR plugin in ImageJ (See software for link). Whilst we have included all experiments and replicates that are included in the paper, data storage constraints meant we could not place our independent repeats of the experiments on this drive. These other replicates can be made available on request. Image sequences from the IR rig are collected into .zip/.7z files which can be opened with a (de)compression program such as 7-zip. These files are organised by Figure.