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Lifespan and ROS levels in different Drosophila melanogaster strains after 24 h hypoxia exposure.

cam.issuedOnline2022-06-29
dc.contributor.authorMalacrida, Sandro
dc.contributor.authorDe Lazzari, Federica
dc.contributor.authorMrakic-Sposta, Simona
dc.contributor.authorVezzoli, Alessandra
dc.contributor.authorZordan, Mauro A
dc.contributor.authorBisaglia, Marco
dc.contributor.authorMenti, Giulio Maria
dc.contributor.authorMeda, Nicola
dc.contributor.authorFrighetto, Giovanni
dc.contributor.authorBosco, Gerardo
dc.contributor.authorDal Cappello, Tomas
dc.contributor.authorStrapazzon, Giacomo
dc.contributor.authorReggiani, Carlo
dc.contributor.authorGussoni, Maristella
dc.contributor.authorMegighian, Aram
dc.contributor.orcidMalacrida, Sandro [0000-0002-4775-3857]
dc.contributor.orcidMeda, Nicola [0000-0003-1114-3632]
dc.date.accessioned2022-06-29T19:50:31Z
dc.date.available2022-06-29T19:50:31Z
dc.date.issued2022-06-15
dc.date.submitted2022-04-11
dc.date.updated2022-06-29T19:50:31Z
dc.descriptionFunder: EURAC Research: Accademia Europea; Grant(s): 58006
dc.description.abstractDuring recent decades, model organisms such as Drosophila melanogaster have made it possible to study the effects of different environmental oxygen conditions on lifespan and oxidative stress. However, many studies have often yielded controversial results usually assigned to variations in Drosophila genetic background and differences in study design. In this study, we compared longevity and ROS levels in young, unmated males of three laboratory wild-type lines (Canton-S, Oregon-R and Berlin-K) and one mutant line (Sod1n1) as a positive control of redox imbalance, under both normoxic and hypoxic (2% oxygen for 24 h) conditions. Lifespan was used to detect the effects of hypoxic treatment and differences were analysed by means of Kaplan-Meier survival curves and log-rank tests. Electron paramagnetic resonance spectroscopy was used to measure ROS levels and analysis of variance was used to estimate the effects of hypoxic treatment and to assess ROS differences between strains. We observed that the genetic background is a relevant factor involved in D. melanogaster longevity and ROS levels. Indeed, as expected, in normoxia Sod1n1 are the shortest-lived, while the wild-type strains, despite a longer lifespan, show some differences, with the Canton-S line displaying the lowest mortality rate. After hypoxic stress these variances are amplified, with Berlin-K flies showing the highest mortality rate and most evident reduction of lifespan. Moreover, our analysis highlighted differential effects of hypoxia on redox balance/unbalance. Canton-S flies had the lowest increase of ROS level compared to all the other strains, confirming it to be the less sensitive to hypoxic stress. Sod1n1 flies displayed the highest ROS levels in normoxia and after hypoxia. These results should be used to further standardize future Drosophila research models designed to investigate genes and pathways that may be involved in lifespan and/or ROS, as well as comparative studies on specific mutant strains.
dc.identifier.doi10.17863/CAM.86022
dc.identifier.eissn2046-6390
dc.identifier.issn2046-6390
dc.identifier.otherbio059386
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/338609
dc.languageen
dc.language.isoeng
dc.publisherThe Company of Biologists
dc.publisher.urlhttp://dx.doi.org/10.1242/bio.059386
dc.subjectDrosophila melanogaster
dc.subjectEPR
dc.subjectHypoxia
dc.subjectLifespan
dc.subjectROS
dc.subjectWild-type strain
dc.subjectAnimals
dc.subjectDrosophila
dc.subjectDrosophila melanogaster
dc.subjectHypoxia
dc.subjectLongevity
dc.subjectMale
dc.subjectOxygen
dc.subjectReactive Oxygen Species
dc.titleLifespan and ROS levels in different Drosophila melanogaster strains after 24 h hypoxia exposure.
dc.typeArticle
dcterms.dateAccepted2022-05-18
prism.issueIdentifier6
prism.publicationNameBiol Open
prism.volume11
pubs.funder-project-idDepartment of Innovation, Research, University and Museums of the Autonomous Province of Bozen/Bolzano (8894/2022)
rioxxterms.licenseref.urihttp://creativecommons.org/licenses/by/4.0
rioxxterms.versionVoR
rioxxterms.versionofrecord10.1242/bio.059386

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