Research data supporting "Deuterium magnetic resonance imaging of serine metabolism in mouse models of glioblastoma"
Repository URI
Repository DOI
Type
Change log
Authors
Description
Zip folder with 7 Excel files containing the raw measurements of
Figure 2_C_D_raw (2C-D Deuterated serine, formate, 5,10‑methylene-THF, glycine and water concentrations in medium from (C) A11 and (D) S2 cells. DMSO-d6 was used as a concentration standard.),
Figure 3_raw (SHMT1,2 and MTHFD1/2 expression in A11 and S2 tumor extracts were assessed by quantitative real-time PCR. For normalization, Ct values of beta-actin were subtracted from Ct values of SHMT1,2 and MTHFD1/2.),
Figure 4_raw (2H NMR measurements of [2H]formate (A), HDO (B), [2H]glycine (C), [14,14-2H2] 5,10-methylene tetrahydrofolate (D) and [2,3,3-2H2]serine (E) concentrations in S2 cell culture medium 5 h after the addition of 5 mM [2,3,3-2H3]serine and in the presence and absence (untreated) of SHMT and MTHDF2 inhibitors. Pearson correlation analysis of HDO and formate concentrations (F). Two-tailed, Pearson p and r values are shown),
Figure 5_raw (Representative 2H MR spectra of labelled serine and water in A11 (A-C) and S2 (G-I) tumors before, and 20 and 60 min post- [2,3,3-2H3]serine injection. The HDO and serine peaks were fitted individually using prior knowledge, as described in Materials and Methods. Spectroscopic measurements of HDO concentrations in A11 and S2 tumors and in the brains of non-tumor-bearing controls following [2,3,3-2H3]serine injection (1 g/kg) (M). Rate of labeled water production in the brains of non-tumor-bearing control mice and in A11 and S2 tumors (N). [2,3,3-2H3] serine concentrations in A11 and S2 tumors and in the brains of non-tumor-bearing controls following [2,3,3-2H3]serine injection (1 g/kg) (O).),
Figure 6C_raw (Concentrations of [2,3,3-2H3]serine, [2H]formate and [14,14-2H2] 5,10‑methylene-THF from 2H NMR spectra of A11 and S2 tumor extracts were determined by fitting their resonances to Lorenztian lineshapes and taking the integrals.).
Supplementary_Figure_1_raw (1B Protein expression levels of TOMM20 were evaluated in S2 and A11 tumors by western blot and band intensities relative to GAPDH determined by densitometry.), Supplementary_Figure_2_raw (Expression of amino acid transporter genes in A11 and S2 cells determined by RNA sequencing.)