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Genomic analysis finds no evidence of canonical eukaryotic DNA processing complexes in a free-living protist.

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cam.issuedOnline2021-10-14
dc.contributor.authorSalas-Leiva, Dayana E
dc.contributor.authorTromer, Eelco C
dc.contributor.authorCurtis, Bruce A
dc.contributor.authorJerlström-Hultqvist, Jon
dc.contributor.authorKolisko, Martin
dc.contributor.authorYi, Zhenzhen
dc.contributor.authorSalas-Leiva, Joan S
dc.contributor.authorGallot-Lavallée, Lucie
dc.contributor.authorWilliams, Shelby K
dc.contributor.authorKops, Geert JPL
dc.contributor.authorArchibald, John M
dc.contributor.authorSimpson, Alastair GB
dc.contributor.authorRoger, Andrew J
dc.contributor.orcidSalas-Leiva, Dayana E [0000-0003-2356-3351]
dc.contributor.orcidTromer, Eelco C [0000-0003-3540-7727]
dc.contributor.orcidCurtis, Bruce A [0000-0001-6729-2173]
dc.contributor.orcidJerlström-Hultqvist, Jon [0000-0002-7992-7970]
dc.contributor.orcidKolisko, Martin [0000-0003-0600-1867]
dc.contributor.orcidYi, Zhenzhen [0000-0002-0693-9989]
dc.contributor.orcidSalas-Leiva, Joan S [0000-0002-4141-140X]
dc.contributor.orcidGallot-Lavallée, Lucie [0000-0001-6763-3388]
dc.contributor.orcidWilliams, Shelby K [0000-0001-7005-5208]
dc.contributor.orcidKops, Geert JPL [0000-0003-3555-5295]
dc.contributor.orcidArchibald, John M [0000-0001-7255-780X]
dc.contributor.orcidSimpson, Alastair GB [0000-0002-4133-1709]
dc.contributor.orcidRoger, Andrew J [0000-0003-1370-9820]
dc.date.accessioned2022-01-05T16:28:16Z
dc.date.available2022-01-05T16:28:16Z
dc.date.issued2021-10-14
dc.date.submitted2021-04-15
dc.date.updated2022-01-05T16:28:14Z
dc.descriptionFunder: Canadian Institutes of Health Research (Grant: FRN-142349) Natural Sciences and Engineering Research Council of Canada (Grant: RGPIN 05871-2014)
dc.description.abstractCells replicate and segregate their DNA with precision. Previous studies showed that these regulated cell-cycle processes were present in the last eukaryotic common ancestor and that their core molecular parts are conserved across eukaryotes. However, some metamonad parasites have secondarily lost components of the DNA processing and segregation apparatuses. To clarify the evolutionary history of these systems in these unusual eukaryotes, we generated a genome assembly for the free-living metamonad Carpediemonas membranifera and carried out a comparative genomics analysis. Here, we show that parasitic and free-living metamonads harbor an incomplete set of proteins for processing and segregating DNA. Unexpectedly, Carpediemonas species are further streamlined, lacking the origin recognition complex, Cdc6 and most structural kinetochore subunits. Carpediemonas species are thus the first known eukaryotes that appear to lack this suite of conserved complexes, suggesting that they likely rely on yet-to-be-discovered or alternative mechanisms to carry out these fundamental processes.
dc.identifier.doi10.17863/CAM.79480
dc.identifier.eissn2041-1723
dc.identifier.issn2041-1723
dc.identifier.others41467-021-26077-2
dc.identifier.other26077
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/332033
dc.languageen
dc.language.isoeng
dc.publisherSpringer Science and Business Media LLC
dc.publisher.urlhttp://dx.doi.org/10.1038/s41467-021-26077-2
dc.subjectAnimals
dc.subjectBiological Evolution
dc.subjectDNA
dc.subjectEukaryota
dc.subjectEukaryotic Cells
dc.subjectGenome
dc.subjectGenomics
dc.subjectMicrobiology
dc.subjectParasites
dc.subjectProteins
dc.titleGenomic analysis finds no evidence of canonical eukaryotic DNA processing complexes in a free-living protist.
dc.typeArticle
dcterms.dateAccepted2021-09-14
prism.issueIdentifier1
prism.publicationNameNat Commun
prism.volume12
rioxxterms.licenseref.urihttp://creativecommons.org/licenses/by/4.0/
rioxxterms.versionVoR
rioxxterms.versionofrecord10.1038/s41467-021-26077-2

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