A charging infrastructure for electric road freight operations is explored in this paper. The city of Cambridge UK was chosen for demonstration but the same methodology could be used for other cities as well. In particular, the five Park and Ride bus routes, the refuse collection operations and two home delivery operations were investigated. Real-time data about existing operations were collected to define accurate drive cycles. Different vehicles were modelled for each operation and their performance was evaluated over the defined drive cycles. Different charging infrastructures were proposed for each operation. The additional power demand, additional load, capital cost needed and the CO2 emissions savings for each case were calculated. The results were scaled up for the entire city and the implications for the electricity supply network were explored. It was shown that electrification of all road freight operations would increase the city’s power demand and electricity consumption by 6.3% and 8.1% respectively based on current figures. Such a system would cost £56.4 million at today’s prices and would result in accumulated savings of 164 MtCO2 by 2050.