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Construction of Fluorescent Analogs to Follow the Uptake and Distribution of Cobalamin (Vitamin B12) in Bacteria, Worms, and Plants

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cam.issuedOnline2018-05-17
dc.contributor.authorLawrence, AD
dc.contributor.authorNemoto-Smith, E
dc.contributor.authorDeery, E
dc.contributor.authorBaker, JA
dc.contributor.authorSchroeder, S
dc.contributor.authorBrown, DG
dc.contributor.authorTullet, JMA
dc.contributor.authorHoward, MJ
dc.contributor.authorBrown, IR
dc.contributor.authorSmith, AG
dc.contributor.authorBoshoff, HI
dc.contributor.authorBarry, CE
dc.contributor.authorWarren, MJ
dc.contributor.orcidSmith, Alison [0000-0001-6511-5704]
dc.date.accessioned2018-09-19T08:38:21Z
dc.date.available2018-09-19T08:38:21Z
dc.date.issued2018-05-17
dc.description.abstractVitamin B 12 is made by only certain prokaryotes yet is required by a number of eukaryotes such as mammals, fish, birds, worms, and Protista, including algae. There is still much to learn about how this nutrient is trafficked across the domains of life. Herein, we describe ways to make a number of different corrin analogs with fluorescent groups attached to the main tetrapyrrole-derived ring. A further range of analogs were also constructed by attaching similar fluorescent groups to the ribose ring of cobalamin, thereby generating a range of complete and incomplete corrinoids to follow uptake in bacteria, worms, and plants. By using these fluorescent derivatives we were able to demonstrate that Mycobacterium tuberculosis is able to acquire both cobyric acid and cobalamin analogs, that Caenorhabditis elegans takes up only the complete corrinoid, and that seedlings of higher plants such as Lepidium sativum are also able to transport B 12 . Lawrence et al., employed chemical biology approaches to construct a range of fluorescent vitamin B 12 derivatives. They demonstrated that these fluorescent variants can be used to follow intracellular B 12 trafficking in bacteria, including E. coli and M. tuberculosis, the worm C. elegans, and a higher plant (Lepidium sativum).
dc.identifier.doi10.17863/CAM.27708
dc.identifier.eissn2451-9448
dc.identifier.issn2451-9456
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/280336
dc.languageeng
dc.language.isoeng
dc.publisherElsevier
dc.publisher.urlhttps://www.sciencedirect.com/science/article/pii/S2451945618301429?via=ihub#!
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectvitamin B12
dc.subjectcobalamin
dc.subjecttetrapyrrole
dc.subjectbiosynthesis
dc.subjectanalogs
dc.subjectfluorescence
dc.subjecttrafficking
dc.subjectMycobacterium tuberculosis
dc.subjectCaenorhabditis elegans
dc.subjecthigher plants
dc.titleConstruction of Fluorescent Analogs to Follow the Uptake and Distribution of Cobalamin (Vitamin B<inf>12</inf>) in Bacteria, Worms, and Plants
dc.typeArticle
dcterms.dateAccepted2018-04-12
prism.endingPage951.e6
prism.issueIdentifier8
prism.publicationDate2018
prism.publicationNameCell Chemical Biology
prism.startingPage941
prism.volume25
pubs.funder-project-idRoyal Society (unknown)
pubs.funder-project-idBiotechnology and Biological Sciences Research Council (BB/I013164/1)
rioxxterms.licenseref.startdate2018-05-17
rioxxterms.licenseref.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
rioxxterms.typeJournal Article/Review
rioxxterms.versionAM
rioxxterms.versionofrecord10.1016/j.chembiol.2018.04.012

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