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Investigating the interactions of endornaviruses with each other and with other viruses in common bean, Phaseolus vulgaris.

Accepted version
Peer-reviewed

Type

Article

Change log

Authors

Viswanathan, Satish B 
Pate, Adrienne E 

Abstract

BACKGROUND: Plant viruses of the genus Alphaendornavirus are transmitted solely via seed and pollen and generally cause no apparent disease. It has been conjectured that certain plant endornaviruses may confer advantages on their hosts through improved performance (e.g., seed yield) or resilience to abiotic or biotic insult. We recently characterised nine common bean (Phaseolus vulgaris L.) varieties that harboured either Phaseolus vulgaris endornavirus (PvEV1) alone, or PvEV1 in combination with PvEV2 or PvEV1 in combination with PvEV2 and PvEV3. Here, we investigated the interactions of these endornaviruses with each other, and with three infectious pathogenic viruses: cucumber mosaic virus (CMV), bean common mosaic virus (BCMV), and bean common mosaic necrosis virus (BCMNV). RESULTS: In lines harbouring PvEV1, PvEV1 and PvEV2, or PvEV1, PvEV2 plus PvEV3, the levels of PvEV1 and PvEV3 RNA were very similar between lines, although there were variations in PvEV2 RNA accumulation. In plants inoculated with infectious viruses, CMV, BCMV and BCMNV levels varied between lines, but this was most likely due to host genotype differences rather than to the presence or absence of endornaviruses. We tested the effects of endornaviruses on seed production and seedborne transmission of infectious pathogenic viruses but found no consistent relationship between the presence of endornaviruses and seed yield or protection from seedborne transmission of infectious pathogenic viruses. CONCLUSIONS: It was concluded that endornaviruses do not interfere with each other's accumulation. There appears to be no direct synergy or competition between infectious pathogenic viruses and endornaviruses, however, the effects of host genotype may obscure interactions between endornaviruses and infectious viruses. There is no consistent effect of endornaviruses on seed yield or susceptibility to seedborne transmission of other viruses.

Description

Keywords

Persistent virus, Viral cross-protection, Viral mutualism, Viral synergy

Journal Title

Virol J

Conference Name

Journal ISSN

1743-422X
1743-422X

Volume Title

Publisher

BMC
Sponsorship
BBSRC (BB/W510609/1)
Leverhulme Trust (RPG-2022-134)
Biotechnology and Biological Sciences Research Council (BB/J011762/1)
Biotechnology and Biological Sciences Research Council (BB/P023223/1)
Royal Society (FCG\R1\201005)
FOW was supported by a Royal Society Future Leaders – African Independent Research (FLAIR) Fellowship (FLR\R1\190462) and a Royal Society FLAIR Collaboration Grant 2020 (FCG\R1\201005) and grants from the UK Biotechnological and Biological Sciences Research Council (SCPRID grant number BB/J011762/1, GCRF grant number BB/P023223/1, and 21ROMITIGATIONFUND CAMBRIDGE BB/W510609/1) and The Leverhulme Trust (RPG-2022-134). TJB was supported by a Masters Studentship from the British Society for Plant Pathology, and SVB by a studentship from the Cambridge Trusts.