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Circulating intermediate monocytes CD14++CD16+ are increased after elective percutaneous coronary intervention.

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Merinopoulos, Ioannis 
Bhalraam, U 
Holmes, Terri 
Tsampasian, Vasiliki 
Corballis, Natasha 


AIM: Inflammation plays a central role in the pathogenesis of atherosclerosis and in the sequelae of percutaneous coronary intervention (PCI). Previous work demonstrated that intermediate monocytes (CD14++CD16+) are associated with adverse cardiovascular events, yet monocyte subset response following elective PCI has not been described. This article explores the changes in monocyte subset and humoral response after elective PCI. METHODS: This prospective study included 30 patients without inflammatory diseases being referred for elective PCI. We included patients treated with drug coated balloons or 2nd generation drug eluting stents. Patients underwent blood tests at baseline (prior to PCI), four hours, two weeks and two months later. Analyses were performed in terms of monocyte subsets (classical CD14++CD16-, intermediate CD14++CD16+ and non-classical CD14+CD16++), gene expression of CD14+ leucocytes and humoral biomarkers. RESULTS: Intermediate monocytes decreased significantly four hours after PCI, were recovered at two weeks, and increased significantly at two months post elective, uncomplicated PCI. They remain significantly elevated in the DES group but not in the DCB group. Gene expression analysis of CD14+ leucocytes showed IL18 had decreased expression at two weeks, CXCR4 and IL1β decreased at two months, while pentraxin 3 increased at two weeks and two months. In terms of humoral biomarkers, hsTnI remains elevated up to two weeks post PCI while IL6 and TNFα remain elevated till two months post PCI. CONCLUSION: Intermediate monocytes increase significantly two months following elective, uncomplicated PCI. They remain significantly elevated in the DES group but not in the DCB group suggesting that the PCI strategy could be one of the ways to modulate the inflammatory response post PCI.


Acknowledgements: We would like to thank all staff at the cardiology department at the Norfolk & Norwich University Hospital who facilitated this study.

Funder: NIHR

Funder: National Institute for Health Research Capability Fund from Norfolk and Norwich University Hospital

Funder: Norfolk Heart Trust

Funder: B Braun Ltd.


Humans, Monocytes, Prospective Studies, Percutaneous Coronary Intervention, Lipopolysaccharide Receptors, Biomarkers, Inflammation, Receptors, IgG, GPI-Linked Proteins

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PLoS One

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Public Library of Science (PLoS)