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Human skeletal muscle aging atlas.

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Kedlian, Veronika R  ORCID logo
Wang, Yaning 
Chen, Xiaoping 


Skeletal muscle aging is a key contributor to age-related frailty and sarcopenia with substantial implications for global health. Here we profiled 90,902 single cells and 92,259 single nuclei from 17 donors to map the aging process in the adult human intercostal muscle, identifying cellular changes in each muscle compartment. We found that distinct subsets of muscle stem cells exhibit decreased ribosome biogenesis genes and increased CCL2 expression, causing different aging phenotypes. Our atlas also highlights an expansion of nuclei associated with the neuromuscular junction, which may reflect re-innervation, and outlines how the loss of fast-twitch myofibers is mitigated through regeneration and upregulation of fast-type markers in slow-twitch myofibers with age. Furthermore, we document the function of aging muscle microenvironment in immune cell attraction. Overall, we present a comprehensive human skeletal muscle aging resource ( ) together with an in-house mouse muscle atlas to study common features of muscle aging across species.



Humans, Aging, Muscle, Skeletal, Animals, Mice, Adult, Aged, Sarcopenia, Male, Neuromuscular Junction, Middle Aged, Female

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Nat Aging

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Springer Science and Business Media LLC
Wellcome Trust (Wellcome) (WT211276/Z/18/Z, WT206194)
Wellcome Trust (Wellcome) (220540/Z/20/A)
National Natural Science Foundation of China (National Science Foundation of China) (32000840, 31871370)
Natural Science Foundation of Guangdong Province (Guangdong Natural Science Foundation) (2021A1515012065)
EC | Horizon 2020 Framework Programme (EU Framework Programme for Research and Innovation H2020) (101026233)