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Responsive core-shell DNA particles trigger lipid-membrane disruption and bacteria entrapment

Published version
Peer-reviewed

Change log

Authors

Brady, Ryan A. 
Mancini, Leonardo 
Rubio-Sánchez, Roger  ORCID logo  https://orcid.org/0000-0001-5574-5809

Abstract

Abstract: Biology has evolved a variety of agents capable of permeabilizing and disrupting lipid membranes, from amyloid aggregates, to antimicrobial peptides, to venom compounds. While often associated with disease or toxicity, these agents are also central to many biosensing and therapeutic technologies. Here, we introduce a class of synthetic, DNA-based particles capable of disrupting lipid membranes. The particles have finely programmable size, and self-assemble from all-DNA and cholesterol-DNA nanostructures, the latter forming a membrane-adhesive core and the former a protective hydrophilic corona. We show that the corona can be selectively displaced with a molecular cue, exposing the ‘sticky’ core. Unprotected particles adhere to synthetic lipid vesicles, which in turn enhances membrane permeability and leads to vesicle collapse. Furthermore, particle-particle coalescence leads to the formation of gel-like DNA aggregates that envelop surviving vesicles. This response is reminiscent of pathogen immobilisation through immune cells secretion of DNA networks, as we demonstrate by trapping E. coli bacteria.

Description

Funder: Wellcome Trust (Wellcome); doi: https://doi.org/10.13039/100004440

Keywords

Article, /639/638/298/54/989, /639/925/926/1049, /639/925/926/1050, /147, /123, /147/143, /128, /132, article

Journal Title

Nature Communications

Conference Name

Journal ISSN

2041-1723

Volume Title

12

Publisher

Nature Publishing Group UK
Sponsorship
RCUK | Engineering and Physical Sciences Research Council (EPSRC) (EP/T002778, EP/T002778)
EC | EU Framework Programme for Research and Innovation H2020 | H2020 Euratom (H2020 Euratom Research and Training Programme 2014-2018) (851667)
Royal Society (UF160152)