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Sequences in the cytoplasmic tail of SARS-CoV-2 Spike facilitate expression at the cell surface and syncytia formation.

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Cattin-Ortolá, Jérôme  ORCID logo
Papa, Guido 


The Spike (S) protein of SARS-CoV-2 binds ACE2 to direct fusion with host cells. S comprises a large external domain, a transmembrane domain, and a short cytoplasmic tail. Understanding the intracellular trafficking of S is relevant to SARS-CoV-2 infection, and to vaccines expressing full-length S from mRNA or adenovirus vectors. Here we report a proteomic screen for cellular factors that interact with the cytoplasmic tail of S. We confirm interactions with the COPI and COPII vesicle coats, ERM family actin regulators, and the WIPI3 autophagy component. The COPII binding site promotes exit from the endoplasmic reticulum, and although binding to COPI should retain S in the early Golgi where viral budding occurs, there is a suboptimal histidine residue in the recognition motif. As a result, S leaks to the surface where it accumulates and can direct the formation of multinucleate syncytia. Thus, the trafficking signals in the tail of S indicate that syncytia play a role in the SARS-CoV-2 lifecycle.



Angiotensin-Converting Enzyme 2, Animals, COP-Coated Vesicles, COVID-19, Cell Membrane, Chlorocebus aethiops, Endoplasmic Reticulum, Giant Cells, Golgi Apparatus, HEK293 Cells, Humans, Protein Binding, Protein Domains, Proteomics, SARS-CoV-2, Spike Glycoprotein, Coronavirus, Vero Cells, Virus Assembly

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Nat Commun

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Springer Science and Business Media LLC
RCUK | Medical Research Council (MC_U105178783)
Medical Research Council (MC_U105178783)