Repository logo
 

Research data supporting "Design, development, and validation of a strand-specific RT-qPCR assay for GI and GII human Noroviruses"

Repository URI

https://www.repository.cam.ac.uk/handle/1810/327387

Repository DOI

https://doi.org/10.17863/CAM.74820

Files

Fig.1c RNA standards GI raw.jpg (9.94 KB)
 Fig.1c RNA standards GII raw.jpg (20.41 KB)
 Fig.2d qPCR gel raw.jpg (8.44 KB)
 Fig.2c qPCR gel raw.jpg (8.95 KB)
 Fig.3c qPCR gel raw.jpg (8.73 KB)

Type

Dataset

Change log

Authors

König, Katja Marie Kjara 
Nayak, Komal 
Drumright, Lydia 
Show 2 more

Description

  1. raw images collected directly gel-doc imager
  2. raw qPCR data files exported from Viia7 or StepOnePlus qPCR machine
  3. sequence alignment files for calivirus strains presented in the manuscript

Version

Software / Usage instructions

ViiATM7 software v1.1: embedded in ViiA 7 real time PCR machine AliView version 1.26: used to align sequences. All gene accession number were described in the manuscript

Keywords

gel-doc images, RT-qPCR, strand-specific RT-qPCR, human norovirus, caliciviruses

Publisher

Rights

Attribution 4.0 International (CC BY 4.0) Repository logo
Sponsorship
Wellcome Trust (097997/Z/11/Z)
Wellcome Trust Senior Fellowship (207498/Z/17/Z) Erasmus visiting studentship MRC New Investigator Research Grant (MR/T001917/1, Award number: RG99899)
Relationships
Supplements:
https://www.repository.cam.ac.uk/handle/1810/330257
https://doi.org/10.12688/wellcomeopenres.17078.1

Collections

Research Data - Pathology