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Functional connexin35 increased in the myopic chicken retina.

Published version
Peer-reviewed

Type

Article

Change log

Authors

Banerjee, Seema 
Wang, Qing 
Tang, George 
So, ChungHim 
Shan, Sze Wan 

Abstract

Our previous research showed that increased phosphorylation of connexin (Cx)36 indicated extended  coupling of AII amacrine cells (ACs) in the rod-dominant mouse myopic retina. This research will determine whether phosphorylation at serine 276 of Cx35-containing gap junctions increased in the myopic chicken, whose retina is cone-dominant. Refractive errors and ocular biometric dimensions of 7-days-old chickens were determined following 12 h and 7 days induction of myopia by a -10D lens. The expression pattern and size of Cx35-positive plaques were examined in the early (12 h) and compensated stages (7 days) of lens-induced myopia (LIM). At the same time, phosphorylation at serine 276 (functional assay) of Cx35 in strata 5 (S5) of the inner plexiform layer was investigated. The axial length of the 7 days LIM eyes was significantly longer than that of non-LIM controls (P < 0.05). Anti-phospho-Ser276 (Ser276-P)-labeled plaques were significantly increased in LIM retinas at both 12 h and 7 days. The density of Ser276-P of Cx35 was observed to increase after 12 h LIM. In the meanwhile, the areas of existing Cx35 plaques did not change. As there was more phosphorylation of connexin35 at Ser276 at both the early and late stages (12 h) and 7 days of LIM chicken retinal activity, the coupling with ACs could be increased in myopia development of the cone-dominated chicken retina.

Description

Keywords

amacrine cell, gap junction, myopia, retina, Animals, Chickens, Gap Junctions, Mice, Myopia, Retina, Retinal Cone Photoreceptor Cells

Journal Title

Vis Neurosci

Conference Name

Journal ISSN

0952-5238
1469-8714

Volume Title

38

Publisher

Cambridge University Press (CUP)