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In vivo, genome-wide profiling of endogenously tagged chromatin-binding proteins with spatial and temporal resolution using NanoDam in Drosophila.

Published version
Peer-reviewed

Type

Article

Change log

Authors

Tang, Jocelyn LY 
Llorà-Batlle, Oriol  ORCID logo  https://orcid.org/0000-0002-8424-9705
Hakes, Anna E 
Fox, Paul M 

Abstract

NanoDam is a technique for genome-wide profiling of the binding targets of any endogenously tagged chromatin-binding protein in vivo, without the need for antibodies, crosslinking, or immunoprecipitation. Here, we explain the procedure for NanoDam experiments in Drosophila, starting from a genetic cross, to the generation of sequencing libraries and, finally, bioinformatic analysis. This protocol can be readily adapted for use in other model systems after simple modifications. For complete details on the use and execution of this protocol, please refer to Tang et al. (2022).

Description

Keywords

Cell biology, Developmental biology, Genomics, Model organisms, Sequencing, Animals, Chromatin, Chromatin Immunoprecipitation, Drosophila, Carrier Proteins, High-Throughput Nucleotide Sequencing

Journal Title

STAR Protoc

Conference Name

Journal ISSN

2666-1667
2666-1667

Volume Title

3

Publisher

Elsevier BV
Sponsorship
Royal Society (RP150061)
Wellcome Trust (092545/Z/10/Z)
Wellcome Trust (103792/Z/14/Z)
Wellcome Trust (092096/Z/10/Z)
Cancer Research Uk (None)
Wellcome Trust (102454/Z/13/Z)
Wellcome Trust (203798/Z/16/Z)