Identification, Characterization, and Heritability of Murine Metastable Epialleles: Implications for Non-genetic Inheritance.
cam.issuedOnline | 2018-10-25 | |
dc.contributor.author | Kazachenka, Anastasiya | |
dc.contributor.author | Bertozzi, Tessa M | |
dc.contributor.author | Sjoberg-Herrera, Marcela K | |
dc.contributor.author | Walker, Nic | |
dc.contributor.author | Gardner, Joseph | |
dc.contributor.author | Gunning, Richard | |
dc.contributor.author | Pahita, Elena | |
dc.contributor.author | Adams, Sarah | |
dc.contributor.author | Adams, David | |
dc.contributor.author | Ferguson-Smith, Anne C | |
dc.contributor.orcid | Ferguson-Smith, Anne [0000-0003-4996-9990] | |
dc.date.accessioned | 2018-11-22T00:31:38Z | |
dc.date.available | 2018-11-22T00:31:38Z | |
dc.date.issued | 2018-11-15 | |
dc.description.abstract | Generally repressed by epigenetic mechanisms, retrotransposons represent around 40% of the murine genome. At the Agouti viable yellow (Avy) locus, an endogenous retrovirus (ERV) of the intracisternal A particle (IAP) class retrotransposed upstream of the agouti coat-color locus, providing an alternative promoter that is variably DNA methylated in genetically identical individuals. This results in variable expressivity of coat color that is inherited transgenerationally. Here, a systematic genome-wide screen identifies multiple C57BL/6J murine IAPs with Avy epigenetic properties. Each exhibits a stable methylation state within an individual but varies between individuals. Only in rare instances do they act as promoters controlling adjacent gene expression. Their methylation state is locus-specific within an individual, and their flanking regions are enriched for CTCF. Variably methylated IAPs are reprogrammed after fertilization and re-established as variable loci in the next generation, indicating reconstruction of metastable epigenetic states and challenging the generalizability of non-genetic inheritance at these regions. | |
dc.format.medium | Print-Electronic | |
dc.identifier.doi | 10.17863/CAM.32982 | |
dc.identifier.eissn | 1097-4172 | |
dc.identifier.issn | 0092-8674 | |
dc.identifier.uri | https://www.repository.cam.ac.uk/handle/1810/285629 | |
dc.language | eng | |
dc.language.iso | eng | |
dc.publisher | Elsevier BV | |
dc.publisher.url | http://dx.doi.org/10.1016/j.cell.2018.09.043 | |
dc.rights | Attribution 4.0 International | |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | |
dc.subject | Agouti Signaling Protein | |
dc.subject | Animals | |
dc.subject | Binding Sites | |
dc.subject | CCCTC-Binding Factor | |
dc.subject | DNA Methylation | |
dc.subject | Epigenesis, Genetic | |
dc.subject | Genes, Intracisternal A-Particle | |
dc.subject | Genetic Loci | |
dc.subject | Genome | |
dc.subject | Genomic Instability | |
dc.subject | Heredity | |
dc.subject | Male | |
dc.subject | Mice | |
dc.subject | Mice, Inbred C57BL | |
dc.subject | Protein Binding | |
dc.subject | Retroelements | |
dc.subject | Transcription, Genetic | |
dc.title | Identification, Characterization, and Heritability of Murine Metastable Epialleles: Implications for Non-genetic Inheritance. | |
dc.type | Article | |
dcterms.dateAccepted | 2018-09-19 | |
prism.endingPage | 1271.e13 | |
prism.issueIdentifier | 5 | |
prism.publicationDate | 2018 | |
prism.publicationName | Cell | |
prism.startingPage | 1259 | |
prism.volume | 175 | |
pubs.funder-project-id | Medical Research Council (MR/J001597/1) | |
pubs.funder-project-id | Wellcome Trust (086838/Z/08/Z) | |
pubs.funder-project-id | Wellcome Trust (095606/Z/11/Z) | |
rioxxterms.licenseref.startdate | 2018-11 | |
rioxxterms.licenseref.uri | http://www.rioxx.net/licenses/all-rights-reserved | |
rioxxterms.type | Journal Article/Review | |
rioxxterms.version | VoR | |
rioxxterms.versionofrecord | 10.1016/j.cell.2018.09.043 |
Files
Original bundle
1 - 1 of 1
Loading...
- Name:
- 1-s2.0-S0092867418312558-main.pdf
- Size:
- 3.32 MB
- Format:
- Adobe Portable Document Format
- Description:
- Published version
- Licence
- https://creativecommons.org/licenses/by/4.0/
License bundle
1 - 1 of 1
No Thumbnail Available
- Name:
- DepositLicenceAgreementv2.1.pdf
- Size:
- 150.9 KB
- Format:
- Adobe Portable Document Format