B cell receptor repertoire kinetics after SARS-CoV-2 infection and vaccination.


Type
Article
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Authors
Kotagiri, Prasanti 
Mescia, Federica 
Rae, William M 
Bergamaschi, Laura 
Abstract

B cells are important in immunity to both severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) infection and vaccination, but B cell receptor (BCR) repertoire development in these contexts has not been compared. We analyze serial samples from 171 SARS-CoV-2-infected individuals and 63 vaccine recipients and find the global BCR repertoire differs between them. Following infection, immunoglobulin (Ig)G1/3 and IgA1 BCRs increase, somatic hypermutation (SHM) decreases, and, in severe disease, IgM and IgA clones are expanded. In contrast, after vaccination, the proportion of IgD/M BCRs increase, SHM is unchanged, and expansion of IgG clones is prominent. VH1-24, which targets the N-terminal domain (NTD) and contributes to neutralization, is expanded post infection except in the most severe disease. Infection generates a broad distribution of SARS-CoV-2-specific clones predicted to target the spike protein, while a more focused response after vaccination mainly targets the spike's receptor-binding domain. Thus, the nature of SARS-CoV-2 exposure differentially affects BCR repertoire development, potentially informing vaccine strategies.

Description
Keywords
B cell receptor repertoire, COVID-19, SARS-CoV-2 vaccination
Journal Title
Cell Reports
Conference Name
Journal ISSN
2211-1247
2211-1247
Volume Title
Publisher
Elsevier
Rights
Publisher's own licence
Sponsorship
Wellcome Trust (200871/Z/16/Z)
Medical Research Council (MR/P008801/1)
Wellcome Trust (207498/Z/17/Z)
Medical Research Council (MR/S035842/1)