FeII 4L4 tetrahedron binds and aggregates DNA G-quadruplexes.
| cam.issuedOnline | 2021-10-07 | |
| dc.contributor.author | Zhu, Jinbo | |
| dc.contributor.author | Yan, Zhiqiang | |
| dc.contributor.author | Bošković, Filip | |
| dc.contributor.author | Haynes, Cally JE | |
| dc.contributor.author | Kieffer, Marion | |
| dc.contributor.author | Greenfield, Jake L | |
| dc.contributor.author | Wang, Jin | |
| dc.contributor.author | Nitschke, Jonathan R | |
| dc.contributor.author | Keyser, Ulrich F | |
| dc.contributor.orcid | Zhu, Jinbo [0000-0003-4935-825X] | |
| dc.contributor.orcid | Yan, Zhiqiang [0000-0003-3865-3229] | |
| dc.contributor.orcid | Bošković, Filip [0000-0001-7663-2408] | |
| dc.contributor.orcid | Haynes, Cally JE [0000-0003-4262-8560] | |
| dc.contributor.orcid | Kieffer, Marion [0000-0003-0202-7445] | |
| dc.contributor.orcid | Greenfield, Jake L [0000-0002-7650-5414] | |
| dc.contributor.orcid | Wang, Jin [0000-0002-2841-4913] | |
| dc.contributor.orcid | Nitschke, Jonathan R [0000-0002-4060-5122] | |
| dc.contributor.orcid | Keyser, Ulrich F [0000-0003-3188-5414] | |
| dc.date.accessioned | 2022-01-28T14:44:59Z | |
| dc.date.available | 2022-01-28T14:44:59Z | |
| dc.date.issued | 2021-11-10 | |
| dc.date.updated | 2022-01-28T14:44:58Z | |
| dc.description.abstract | Since the discovery of the G-quadruplex (G4) structure in telomeres in 1980s, studies have established the role it plays in various biological processes. Here we report binding between DNA G4 and a self-assembled tetrahedral metal-organic cage 1 and consequent formation of aggregates, whereby the cage protects the DNA G4 from cleavage by S1 nuclease. We monitor DNA-cage interaction using fluorescence spectroscopy, firstly by quenching of a fluorescent label appended to the 5' end of G4. Secondly, we detect the decrease in fluorescence of the G4-selective dyes thioflavin-T and Zn-PPIX bound to various DNA G4 sequences following the addition of cage 1. Our results demonstrate that 1 interacts with a wide range of G4s. Moreover, gel electrophoresis, circular dichroism and dynamic light scattering measurements establish the binding of 1 to G4 and indicate the formation of aggregate structures. Finally, we find that DNA G4 contained in an aggregate of cage 1 is protected from cleavage by S1 nuclease. | |
| dc.identifier.doi | 10.17863/CAM.80504 | |
| dc.identifier.eissn | 2041-6539 | |
| dc.identifier.issn | 2041-6520 | |
| dc.identifier.other | 34881008 | |
| dc.identifier.other | PMC8580047 | |
| dc.identifier.uri | https://www.repository.cam.ac.uk/handle/1810/333080 | |
| dc.language | eng | |
| dc.language.iso | eng | |
| dc.publisher | Royal Society of Chemistry (RSC) | |
| dc.publisher.url | http://dx.doi.org/10.1039/d1sc04430c | |
| dc.rights | Attribution 4.0 International | |
| dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | |
| dc.source | nlmid: 101545951 | |
| dc.source | essn: 2041-6539 | |
| dc.subject | 3403 Macromolecular and Materials Chemistry | |
| dc.subject | 34 Chemical Sciences | |
| dc.subject | Genetics | |
| dc.title | FeII 4L4 tetrahedron binds and aggregates DNA G-quadruplexes. | |
| dc.type | Article | |
| dcterms.dateAccepted | 2021-10-05 | |
| prism.endingPage | 14569 | |
| prism.issueIdentifier | 43 | |
| prism.publicationName | Chem Sci | |
| prism.startingPage | 14564 | |
| prism.volume | 12 | |
| pubs.funder-project-id | Engineering and Physical Sciences Research Council (EP/M008258/1) | |
| pubs.funder-project-id | European Research Council (647144) | |
| rioxxterms.licenseref.uri | https://creativecommons.org/licenses/by/4.0/ | |
| rioxxterms.version | VoR | |
| rioxxterms.versionofrecord | 10.1039/d1sc04430c |
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