This paper presents the development of a miniaturized sensor device for selective detection of pathogens, specifically Influenza A Influenza virus, as an enveloped virus is relatively vulnerable to damaging environmental impacts. In consideration of environmental factors such as humidity and temperature, this particular pathogen proves to be an ideal choice for our study. It falls into the category of pathogens that pose greater challenges due to their susceptibility. An impedance biosensor was integrated into an existing platform and effectively separated and detected high concentrations of airborne pathogens. Bio-functionalized hydrogel-based detectors were utilized to analyze virus-containing particles. The sensor device demonstrated high sensitivity and specificity when exposed to varying concentrations of Influenza A virus ranging from 0.5 to 50 μg/mL. The sensitivity of the device for a 0.5 μg/mL analyte concentration was measured to be 695 Ω· mL/μg. Integration of this pathogen detector into a compact-design air quality monitoring device could foster the advancement of personal exposure monitoring applications. The proposed sensor device offers a promising approach for real-time pathogen detection in complex environmental settings.